Regulatory crosstalk between type I and type II toxin-antitoxin systems in the human pathogen Enterococcus faecalis
| Autor: | Aymeric Fouquier d'Hérouël, Francis Repoila, Nathalie Goeders, Françoise Wessner, Renata C. Matos, Caroline Lacoux, Laurence Van Melderen, Pascale Serror |
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| Přispěvatelé: | MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Faculté des Sciences, Institut de Biologie et Médecine Moléculaire, Université Libre de Bruxelles [Bruxelles] (ULB), INRA UMR1319 Micalis, Agence Nationale pour la Recherche ANR-12-BSV6-0008, Université libre de Bruxelles (ULB) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
GRAM-POSITIVE BACTERIA INHIBITS TRANSLATION Bacterial Toxins antisense RNAs medicine.disease_cause DNA-binding protein Enterococcus faecalis BACILLUS-SUBTILIS SELECTIVE TRANSLATION Transcription (biology) Endoribonucleases Escherichia coli medicine Humans RNA Antisense Amino Acid Sequence MESSENGER-RNAS Molecular Biology toxin-antitoxin systems STAPHYLOCOCCUS-AUREUS Genetics biology Escherichia coli Proteins transcription activation RNA Gene Expression Regulation Bacterial Cell Biology RNA stability biology.organism_classification Antisense RNA DNA-Binding Proteins Crosstalk (biology) ESCHERICHIA-COLI NONCODING RNAS PROTEIN-SYNTHESIS Antitoxins Antitoxin CONDITIONAL COOPERATIVITY Research Paper |
| Zdroj: | RNA Biology RNA Biology, Taylor & Francis, 2015, 12 (10), pp.1099-1108. ⟨10.1080/15476286.2015.1084465⟩ |
| ISSN: | 1547-6286 1555-8584 |
| DOI: | 10.1080/15476286.2015.1084465⟩ |
| Popis: | We discovered a chromosomal locus containing 2 toxin-antitoxin modules (TAs) with an antisense transcriptional organization in the E. faecalis clinical isolate V583. These TAs are homologous to the type I txpA-ratA system and the type II mazEF, respectively. We have shown that the putative MazF is toxic for E. coli and triggers RNA degradation, and its cognate antitoxin MazE counteracts toxicity. The second module, adjacent to mazEF, expresses a toxin predicted to belong to the TxpA type I family found in Firmicutes, and the antisense RNA antidote, RatA. Genomic analysis indicates that the cis-association of mazEF and txpA-ratA modules has been favored during evolution, suggesting a selective advantage for this TA organization in the E. faecalis species. We showed regulatory interplays between the 2 modules, involving transcription control and RNA stability. Remarkably, our data reveal that MazE and MazEF have a dual transcriptional activity: they act as autorepressors and activate ratA transcription, most likely in a direct manner. RatA controls txpA RNA levels through stability. Our data suggest a pivotal role of MazEF in the coordinated expression of mazEF and txpA-ratA modules in V583. To our knowledge, this is the first report describing a crosstalk between type I and II TAs. |
| Databáze: | OpenAIRE |
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