Propensity to release endotoxin after two repeated doses of cefuroxime in an in vitro kinetic model: higher release after the second dose
| Autor: | Elisabeth Löwdin, Jan Sjölin, Gunilla Goscinski, Eva Tano |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Microbiology (medical)
Colony Count Microbial Microbial Sensitivity Tests Pharmacology Biology medicine.disease_cause Microbiology Escherichia coli medicine Tobramycin Humans Pharmacology (medical) Antibacterial agent Cefuroxime Protein synthesis inhibitor Dose-Response Relationship Drug Aminoglycoside In vitro Anti-Bacterial Agents Culture Media Endotoxins Kinetics Infectious Diseases Microscopy Electron Scanning Liberation medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 60:328-333 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkm190 |
| Popis: | To study endotoxin release from two strains of Escherichia coli after exposure to two repeated doses of cefuroxime in an in vitro kinetic model.Cefuroxime in concentrations simulating human pharmacokinetics was added to the bacterial solution with a repeated dose after 12 h. In another experiment, tobramycin was given concomitantly with the second dose of cefuroxime. Samples for viable counts and endotoxin analyses were drawn before the addition of antibiotics and at 2 and 4 h after each dose.The propensity to release endotoxin, expressed as log10 endotoxin release (EU)/log10 killed bacteria, was higher after the second than after the first dose, 0.80+/-0.04 and 0.65+/-0.01, respectively, in the ATCC strain and 0.80+/-0.04 and 0.65+/-0.02, respectively, in the clinical strain (P0.001). Endotoxin was released earlier after the second dose (P0.001). Addition of tobramycin at the second dose reduced the endotoxin release in comparison with that of cefuroxime alone (P0.001).The propensity to liberate endotoxin is higher after the second dose of cefuroxime than after the first, resulting in a higher release of endotoxin than expected from bacterial count. The release after the second dose can be reduced by the addition of tobramycin. |
| Databáze: | OpenAIRE |
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