When riverine dissolved organic matter (DOM) meets labile DOM in coastal waters: changes in bacterial community activity and composition

Autor: Camila Fernandez, Fran L. Aparicio, Olivier Pringault, Dominique Lefèvre, Fabien Joux, Cèlia Marrasé, Jocelyne Caparros, Jean-François Ghiglione, Bruno Charrière, Marine Blanchet, Louise Oriol, Christos Panagiotopoulos, Philippe Catala
Přispěvatelé: Laboratoire d'Océanographie Microbienne (LOMIC), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Ecologie des systèmes marins côtiers (Ecosym), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Institute of Marine Sciences / Institut de Ciències del Mar [Barcelona] (ICM), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Laboratoire d'océanographie biologique de Banyuls (LOBB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Ministère de l’Enseignement supérieur et de la Recherche (France), Ministerio de Economía y Competitividad (España), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Aquatic Sciences-Research Across Boundaries
Aquatic Sciences-Research Across Boundaries, 2017, 79 (1), pp.27-43. ⟨10.1007/s00027-016-0477-0⟩
Aquatic Sciences (1015-1621) (Springer Basel Ag), 2017-01, Vol. 79, N. 1, P. 27-43
Aquatic Sciences-Research Across Boundaries, Springer Verlag, 2017, 79 (1), pp.27-43. ⟨10.1007/s00027-016-0477-0⟩
Aquatic Sciences
Artículos CONICYT
CONICYT Chile
instacron:CONICYT
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1015-1621
1420-9055
DOI: 10.1007/s00027-016-0477-0⟩
Popis: Blanchet, Marine ... et al.-- 17 pages, 7 figures, 3 tables, supplementary material https://dx.doi.org/10.1007/s00027-016-0477-0
Heterotrophic bacterial communities in marine environments are exposed to a heterogeneous mixture of dissolved organic compounds with different bioreactivity that may control both their activity and composition. The coastal environment is an example of a mixing area where recalcitrant allochthonous organic matter from rivers can encounter labile organic matter from marine phytoplanktonic blooms. The objective of this study was to explore the effects of mixed qualities of dissolved organic matter (DOM) on bacterial community activity (BCA) and bacterial community composition (BCC) and to test for a priming effect when DOM sources are added in combination. Coastal marine bacterial communities were incubated separately with a mixture of amino acids and with natural riverine DOM or with both sources together for 42 days. Addition of amino acids alone or in combination with riverine DOM led to a similar stimulation of BCA compared to control condition, whereas addition of riverine DOM alone did not modify BCA compared to the control. On the contrary, BCC analyzed by 16S rRNA gene pyrosequencing was not affected by the addition of amino acids alone, but changed dramatically with riverine DOM alone or in combination with amino acids. Our results show that changes in BCA and BCC can be driven by different types of DOM, but that these changes are not necessarily coupled. Moreover, the addition of labile DOM did not modify the microbial decomposition of riverine DOM, nor the BCC, suggesting that a priming effect did not occur under these experimental conditions
This research was funded by the French program EC2CO/CNRS-INSU through the IMPEC project. Rhône River discharge data were provided by MOOSE (Mediterranean Oceanic Observing System on Environment) with the support of the “Agence de l’Eau Rhône-Méditerranée et Corse”. MB was supported by a PhD grant from the French Ministry of Research. C.M. and F.L. Aparicio received support from Ministerio de Economía y Competititvidad (DOREMI project, CTM2012-34294)
Databáze: OpenAIRE