Production of transgenic mice with yeast artificial chromosomes
| Autor: | K R, Peterson, C H, Clegg, Q, Li, G, Stamatoyannopoulos |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Yeast artificial chromosome
Genetics Genetically modified mouse Genetic Vectors Genetic transfer Gene Transfer Techniques Mice Transgenic Locus (genetics) Biology Yeast Globins Mice chemistry.chemical_compound Gene Expression Regulation chemistry hemic and lymphatic diseases Animals Humans Homologous recombination Chromosomes Artificial Yeast Gene DNA |
| Zdroj: | Trends in Genetics. 13:61-66 |
| ISSN: | 0168-9525 |
| Popis: | Techniques are now available that allow the transfer of intact yeast artificial chromosome (YAC) DNA into transgenic mice. Coupled with the ability to perform mutagenesis on YAC sequences by homologous recombination in yeast, they enable the analysis of large genes or multigenic loci in vivo. This system has been used to study the developmental regulation of the human β-globin locus. |
| Databáze: | OpenAIRE |
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