A reproducible porcine ePTFE arterial bypass model for neointimal hyperplasia
| Autor: | Brian Lyle, Samer F. Najjar, Muneera R. Kapadia, Timothy J. Carroll, Jozef Murar, Melina R. Kibbe, Bonnie J. Kane, Oliver Aalami, Jason W.-L. Eng, Patricia M. Cahill, Qun Jiang |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Duplex ultrasonography medicine.medical_specialty Swine Magnetic resonance angiography Restenosis Blood vessel prosthesis medicine Animals Polytetrafluoroethylene Neointimal hyperplasia Ultrasonography Doppler Duplex Hyperplasia medicine.diagnostic_test business.industry Vascular bypass medicine.disease Surgery Blood Vessel Prosthesis Disease Models Animal medicine.anatomical_structure Carotid Arteries Angiography Radiology business Tunica Intima Vascular Surgical Procedures Magnetic Resonance Angiography Artery |
| Zdroj: | The Journal of surgical research. 148(2) |
| ISSN: | 1095-8673 |
| Popis: | Background Late failure of prosthetic vascular bypass grafting using expanded polytetrafluoroethylene (ePTFE) is secondary to the development of neointimal hyperplasia, most commonly at the distal anastomosis. To develop therapies that can improve upon current prosthetic vascular bypass grafting, a large animal model of prosthetic bypass grafting that results in reproducible neointimal hyperplasia is necessary. Methods We performed bilateral end-to-side carotid artery bypasses with 6 mm ePTFE in a porcine model (n = 11). We studied graft patency using magnetic resonance angiography (MRA, 3 wk), duplex ultrasonography (4 wk), and digital-subtraction contrast angiography (4 wk). Animals were sacrificed at 4 wk and morphometric analysis was performed. Results Of the 11 animals that underwent surgery, one pig died from respiratory compromise; of the remaining 10, graft patency was 90% at 4 wk. Peak systolic and end diastolic velocities were established for this model using ultrasonography. MRA, ultrasonography, and angiography confirmed graft patency and were complimentary tools to evaluate the grafts. Development of neointimal hyperplasia was reproducible at 4 wk in both the proximal and distal anastomoses (2.5 to 3 mm2) of the ePTFE bypass grafts. Conclusion We developed a reproducible porcine ePTFE carotid artery bypass model for studying neointimal hyperplasia. Not only does this model allow for the manipulation and evaluation of potential therapies, but patency and neointimal hyperplasia can be easily evaluated by traditional means, such as MRA, ultrasonography, and angiography. This preclinical model is ideal for evaluation of novel therapies in vivo designed to inhibit neointimal hyperplasia following arterial reconstruction with prosthetic bypass grafting. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect