Stress‐specific aggregation of proteins in the amyloid bodies
| Autor: | Monica Luo, Ronnie Tse, Dane Marijan, Sahil Chandhok, Emma Lacroix, Timothy E. Audas, Keenan Elliott |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
Hot Temperature Amyloid Biophysics Protein aggregation Biochemistry Mass Spectrometry Protein Aggregates 03 medical and health sciences Stress Physiological Structural Biology Organelle Genetics Humans Amyloid bodies Molecular Biology 030304 developmental biology Cell Nucleus 0303 health sciences Mechanism (biology) Chemistry 030302 biochemistry & molecular biology Proteins Cell Biology Stimulus exposure Cell biology A549 Cells PC-3 Cells Amyloid aggregation MCF-7 Cells HeLa Cells |
| Zdroj: | FEBS Letters. 593:3162-3172 |
| ISSN: | 1873-3468 0014-5793 |
| Popis: | Physiological amyloid aggregation occurs within the nuclei of stress-treated cells. These structures, termed Amyloid bodies (A-bodies), assemble through the rapid accumulation of proteins into dense membrane-less organelles, which possess the same biophysical properties as plaques observed in many amyloid-based diseases. Here, we demonstrate that A-body proteomic compositions vary significantly between stimuli, as constituent proteins can be sequestered by one or more stressors. Stimulus exposure alone was insufficient to induce aggregation, demonstrating that this pathway is not regulated solely by stress-induced conformational changes of the A-body targets. We propose that different environmental conditions induce the formation of A-body subtypes containing distinct protein residents. This selective immobilization of proteins may have evolved as a finely tuned mechanism for surviving divergent stressors. |
| Databáze: | OpenAIRE |
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