NGF and VEGF effects on retinal ganglion cell fate: new evidence from an animal model of diabetes
| Autor: | Maria Luisa Rocco, Valeria Colafrancesco, Luigi Aloe, Alessandro Lambiase, Flavio Mantelli, Ilaria Macchi |
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| Rok vydání: | 2013 |
| Předmět: |
Retinal degeneration
Blood Glucose Male Retinal Ganglion Cells Vascular Endothelial Growth Factor A medicine.medical_specialty Cell Survival Enzyme-Linked Immunosorbent Assay Tropomyosin receptor kinase A Retina Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine Nerve Growth Factor medicine Animals Receptor trkA Antibodies Blocking Diabetic Retinopathy business.industry Retinal Degeneration General Medicine Anatomy medicine.disease Immunohistochemistry Rats Vascular endothelial growth factor Ophthalmology Vascular endothelial growth factor A Disease Models Animal medicine.anatomical_structure Nerve growth factor Endocrinology Retinal ganglion cell chemistry 030221 ophthalmology & optometry sense organs Ophthalmic Solutions business 030217 neurology & neurosurgery |
| Zdroj: | European journal of ophthalmology. 24(2) |
| ISSN: | 1724-6016 |
| Popis: | To investigate if the survival effects of nerve growth factor (NGF) eyedrops on retinal ganglion cell (RGCs) are related to vascular endothelial growth factor (VEGF) in a rat model of diabetic retinopathy.Diabetes was induced in adult rats by streptozotocin injection and changes in the NGF/TrkA and VEGF retina levels were related to the progression of RGC loss. Diabetic rats were subjected to administration of NGF eyedrops or intraocular injection of anti-NGF antibody. All morphologic, immunohistochemical, and biochemical analyses were performed on whole retinas dissected after 7 or 11 weeks after diabetes induction.Diabetes was successfully induced in rats as shown by glycemic levels250 mg/dL. The NGF levels increased in diabetic retinas at 7 weeks and decreased at 11 weeks, while VEGF levels increased at all time points. The RGC loss in diabetic retinopathy worsened with anti-NGF administration, which did not alter retina VEGF levels significantly. Administration of NGF eyedrops restored TrkA levels in the retina, and protected RGCs from degeneration without influencing VEGF levels.The early increase of NGF in diabetic retina might be an endogenous response for protecting RGCs from degeneration. This protective mechanism is impaired at 11 weeks following diabetes induction, and results in a marked RGC degeneration that is improved by exogenous NGF administration and worsened by anti-NGF. The observed NGF-induced neuroprotection on damaged RGCs was not associated with changes in VEGF retina levels, which were constantly high in diabetic rats and were not altered by anti-NGF administration. |
| Databáze: | OpenAIRE |
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