| Popis: |
This doctment contains supplementary methods and results of the following contents: Firstly, the isolation and conformational analysis of IsoA. Secondly, IsoA inhibited the cologenic formation in a panel of cell lines. Thirdly, IsoA induced ROS in HepG2 cells, and mitochondria should not be the main contribution to IsoA-induced ROS generation. Fourthly, IsoA showed little inhibitory effects on a series of thiol-containing enzymes, including TrxR, PDI, GR, and GST. Fifthly, we showed that Trx1 was overexpressed in cancer cells in parallel with the normal cells lines, and we found that IsoA activated ASK1/JNK signaling pathway. Finally, treatment with IsoA (15 mg/kg/day) displays no obvious toxicity to main organs (liver, kidney, and spleen) in mice by H&E staining. |
