Enantioselective inhibition of human leukocyte elastase
| Autor: | Radhika Venkataraman, Michael A. Stanga, Michael J. Brubaker, William C. Groutas |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
Chymotrypsin biology Pancreatic Elastase Stereochemistry Elastase Biophysics Enantioselective synthesis Succinimides Stereoisomerism Biochemistry chemistry.chemical_compound Kinetics Enzyme chemistry Succinimide Enzyme inhibitor biology.protein Humans Enantiomer Leukocyte Elastase Molecular Biology |
| Zdroj: | Archives of biochemistry and biophysics. 297(1) |
| ISSN: | 0003-9861 |
| Popis: | (RS)-Diethyl-2-benzyl-succinate was resolved using alpha-chymotrypsin. The two enantiomers were then elaborated to yield (S)-(+) and (R)-(-)-3-benzyl-N-[(methyl-sulfonyl)oxy]succinimide and the inhibitory activity of the two enantiomers toward human leukocyte elastase was subsequently determined. The k2/KI values for the R and S isomers were found to be 330 and 1500 M-1 s-1, respectively. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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