iTRAQ analysis of liver immune-related proteins from darkbarbel catfish (Pelteobagrus vachelli) infected with Edwardsiella ictaluri
| Autor: | Xueyin Pei, Jie Li, Shaowu Yin, Tao Wang, Jiejie Xu, Xinyu Zhang, Wu Zhaowen |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Fish Proteins Proteome Aquatic Science Biology 03 medical and health sciences Classical complement pathway Fish Diseases Tandem Mass Spectrometry Environmental Chemistry Animals Proteasome activator complex Edwardsiella ictaluri Catfishes Antigen processing Enterobacteriaceae Infections 04 agricultural and veterinary sciences General Medicine Immunity Innate Complement system 030104 developmental biology Biochemistry Liver 040102 fisheries Alternative complement pathway 0401 agriculture forestry and fisheries Catfish Chromatography Liquid |
| Zdroj: | Fishshellfish immunology. 87 |
| ISSN: | 1095-9947 |
| Popis: | Edwardsiella ictaluri causes enteric septicemia of catfish (ESC), a major disease occurring in these siluriform fish. As the liver is an important organ for defending against bacterial pathogens in fish, this study aimed to determine the liver immune response at the protein level. The differential proteomes of the darkbarbel catfish liver in response to E. ictaluri infection were identified with isobaric tags for relative and absolute quantitation (iTRAQ) labeling followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Using a 1.2-fold change in expression as a physiologically significant benchmark, a total of 819 differentially expressed proteins were reliably quantified using iTRAQ analysis, including 6 up-regulated proteins and 813 down-regulated proteins. GO enrichment analysis indicated that the “complement activation, alternative pathway” and “complement activation, classical pathway” were significantly enriched. KEGG enrichment analysis indicated the “antigen processing and presentation” and “bacterial secretion system” were significantly enriched. We selected the 6 up-regulated proteins and 10 immune-related down-regulated proteins for validation using real-time PCR. The 10 immune-related proteins included complement component C1r, C3, C5, C7, and C9 and plasma protease C1 inhibitor (C1-INH), signal recognition particle 54 kDa protein (SRP54), SRP receptor, proteasome activator complex subunit 1 (PSME1) and major histocompatibility complex class I (MHC class I) were selected from the GO clusters and KEGG pathways. The variations in mRNA expression for these genes were similar to the results of iTRAQ. This is the first report detailing the proteome response in the darkbarbel catfish liver during E. ictaluri infection and markedly contributes to our understanding of the defense mechanisms in the livers of darkbarbel catfish. |
| Databáze: | OpenAIRE |
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