Clonal analysis of Inquilinus limosus isolates from six cystic fibrosis patients and specific serum antibody response
| Autor: | Axel Imhof, Matthias Griese, Michael Hogardt, Juergen Heesemann, Sabine Schmoldt, Philipp Latzin |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) Adolescent Cystic Fibrosis Immunoblotting Respiratory System Inquilinus limosus medicine.disease_cause Microbiology Cystic fibrosis Bacterial Proteins Antigen Antibody Specificity medicine Humans Actinobacillus pleuropneumoniae Alphaproteobacteria biology Pseudomonas aeruginosa General Medicine biology.organism_classification medicine.disease Antibodies Bacterial Random Amplified Polymorphic DNA Technique Molecular Weight Burkholderia Immunoglobulin G Immunology biology.protein Sputum Female medicine.symptom Antibody Gram-Negative Bacterial Infections |
| Zdroj: | Journal of Medical Microbiology. 55:1425-1433 |
| ISSN: | 1473-5644 0022-2615 |
| Popis: | Inquilinus limosus is a novel Gram-negative bacterium of the subdivision α-Proteobacteria recently found in the airways of patients with cystic fibrosis (CF). Here, the authors report on the clinical courses of six CF patients colonized with I. limosus. Five patients suffered from either an acute respiratory exacerbation or a progressive loss of pulmonary function, whereas one patient was in a stable clinical situation. This study focused on two aims: (i) the clonal analysis of I. limosus isolates by random amplified polymorphic DNA (RAPD)-PCR, and (ii) the clarification of whether the presence of I. limosus in the respiratory tract is associated with a specific serum antibody response. Serum IgG was detected by immunoblotting using I. limosus whole-cell-lysate proteins as antigens. Sera from healthy blood donors (n=10) and from CF patients colonized with Pseudomonas aeruginosa (n=10) were found to be immunoblot negative. All six Inquilinus-positive patients raised serum IgG antibodies against various I. limosus antigens. Surprisingly, in one patient, a specific I. limosus serum antibody response was already detected 1 year prior to Inquilinus-positive sputum cultures. Two prominent antigens were characterized by MALDI-MS: a 23 kDa protein revealed homology to the outer membrane lipoprotein OmlA of Actinobacillus pleuropneumoniae, and an 18 kDa protein to a protein-tyrosine phosphatase of Burkholderia cepacia. In conclusion, detection of I. limosus is accompanied by a specific serum antibody response and may reflect the infectious/pathogenic potential of I. limosus. Moreover, IgG immunoblotting may be useful to detect early infection with I. limosus and may support the selective cultivation of this novel emerging pathogen. |
| Databáze: | OpenAIRE |
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