Evaluating the preservation and isolation of stool pathogens using the COPAN FecalSwab™ Transport System and Walk-Away Specimen Processor
Autor: | Tony Mazzulli, Lee W. Goneau, Xhermi Trimi, Anthony Mazzulli, Ana Cabrera, Pauline Lo |
---|---|
Rok vydání: | 2019 |
Předmět: |
Automation
Laboratory Microbiology (medical) Serotype Fastidious organism Bacteriological Techniques Fecal flora Bacteria biology Temperature Bacterial Infections General Medicine biology.organism_classification Isolation (microbiology) Culture Media Gastroenteritis Specimen Handling Microbiology Feces Infectious Diseases Salmonella enterica Pathogen Transport system |
Zdroj: | Diagnostic Microbiology and Infectious Disease. 94:15-21 |
ISSN: | 0732-8893 |
Popis: | The isolation of stool pathogens is difficult due to their fastidious nature and the rapid overgrowth of fecal flora. In this study, we evaluate the preservation and isolation of enteric pathogens from stool using the automated COPAN Walk-Away Specimen Processor (WASP®) in conjunction with FecalSwab™ and selenite media. Pathogen viability and fecal commensal abundance were stable in FecalSwab™ media under both room-temperature and refrigerated incubation conditions, resulting in a significantly increased number of well-isolated pathogen colonies observed when compared to samples incubated in modified Cary-Blair media. Isolation of individual pathogen colonies was improved via WASP® planting compared to those planted using the Isoplater system. Furthermore, preincubation using the newly formulated COPAN selenite media significantly enhanced the yield of Salmonella enterica serovar Typhimurium. Together, the automated WASP® system combined with FecalSwab™ and selenite media represents a rapid and efficient approach for the processing of stool specimens compared to standard methods. |
Databáze: | OpenAIRE |
Externí odkaz: |