Determination of Cancer Cell-Based pH-Sensitive Fluorescent Carbon Nanoparticles of Cross-Linked Polydopamine by Fluorescence Sensing of Alkaline Phosphatase Activity on Coated Surfaces and Aqueous Solution
| Autor: | Zihnil Adha Islamy Mazrad, Sung Han Kim, Insik In, Cheong A Choi, Eun Bi Kang, Sung Young Park |
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| Rok vydání: | 2017 |
| Předmět: |
Indoles
Polymers Surface Properties Diol Inorganic chemistry 02 engineering and technology Conjugated system 010402 general chemistry 01 natural sciences Fluorescence Madin Darby Canine Kidney Cells Analytical Chemistry Nitrophenols chemistry.chemical_compound Hydrolysis Dogs Organophosphorus Compounds Cell Line Tumor Animals Humans Particle Size Fluorescent Dyes Aqueous solution Quenching (fluorescence) beta-Cyclodextrins Water Hydrogen-Ion Concentration Alkaline Phosphatase 021001 nanoscience & nanotechnology Carbon 0104 chemical sciences Solutions Cross-Linking Reagents chemistry Nanoparticles Alkaline phosphatase 0210 nano-technology Boronic acid |
| Zdroj: | Analytical Chemistry. 89:13508-13517 |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.7b03853 |
| Popis: | The tumor-specific sensitive fluorescence sensing of cellular alkaline phosphatase (ALP) activity on the basis of host-guest specific and pH sensitivity was conducted on coated surfaces and aqueous states. Cross-linked fluorescent nanoparticles (C-FNP) consisting of β-cyclodextrin (β-CD)/boronic acid (BA) and fluorescent hyaluronic acid [FNP(HA)] were conjugated to fluorescent polydopamine [FNP(pDA)]. To determine the quenching effect of this system, hydrolysis of 4-nitrophenyl phosphate (NPP) to 4-nitrophenol (NP) was performed in the cavity of β-CD in the presence of ALP activated photoinduced electron transfer (PET) between NP and C-FNP. At an ALP level of 30-1000 U/L, NP caused off-emission of C-FNP because of their specific host-guest recognition. Fluorescence can be recovered under pH shock due to cleavage of the diol bond between β-CD and BA, resulting in release of NP from the fluorescent system. Sensitivity of the assays was assessed by confocal imaging not only in aqueous states, but also for the first time on coated surfaces in MDAMB-231 and MDCK cells. This novel system demonstrated high sensitivity to ALP through generation of good electron donor/acceptor pair during the PET process. Therefore, this fluorescence sensor system can be used to enhance ALP monitoring and cancer diagnosis on both coated surfaces and in aqueous states in clinical settings. |
| Databáze: | OpenAIRE |
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