Mouse polyomavirus large T antigen inhibits cell growth and alters cell and colony morphology inSaccharomyces cerevisiae
| Autor: | T. Adamec, Jitka Forstová, Zdena Palková |
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| Rok vydání: | 2003 |
| Předmět: |
Antigens
Polyomavirus Transforming Saccharomyces cerevisiae Cell Biophysics Cell morphology Biochemistry Cyclic adenosine monophosphate Mice chemistry.chemical_compound Antigen Expression in Saccharomyces cerevisiae Structural Biology Cyclic AMP Genetics medicine Animals Fluorescent Antibody Technique Indirect Molecular Biology Polyomavirus large tumor antigen biology Cell growth Cell Biology biology.organism_classification Molecular biology Recombinant Proteins Yeast medicine.anatomical_structure Microscopy Fluorescence chemistry Growth inhibition Cell Division |
| Zdroj: | FEBS Letters. 555:268-273 |
| ISSN: | 0014-5793 |
| Popis: | The gene for mouse polyomavirus large tumor (LT) antigen, a potent oncoprotein, was expressed in Saccharomyces cerevisiae from the inducible GAL1 promoter. Substantial cell growth inhibition as well as colony and cell morphology changes dependent on cyclic adenosine monophosphate (cAMP) were observed. In contrast to cell and colony morphology alterations, the growth inhibition appeared to be transient, thus indicating the existence of an active adaptation of yeast cells to the LT antigen presence. |
| Databáze: | OpenAIRE |
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