Stearidonic acid promotes insulin secretion via stimulation of G protein-coupled receptor 40 in MIN6 pancreatic β-cells
| Autor: | Jian Zhang, Yixiong Gao, Xin Tan, Cuihong Fan, Jingzhong Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system N-3 polyunsaturated fatty acid Medicine (miscellaneous) Stimulation G protein-coupled receptor 40 MIN6 pancreatic β-cell 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Internal medicine Free fatty acid receptor 1 medicine Insulin TX341-641 Receptor G protein-coupled receptor 030109 nutrition & dietetics Nutrition and Dietetics Phospholipase C Chemistry Nutrition. Foods and food supply 04 agricultural and veterinary sciences 040401 food science Cytosol Endocrinology Stearidonic acid Signal transduction Food Science |
| Zdroj: | Journal of Functional Foods, Vol 60, Iss, Pp-(2019) |
| ISSN: | 1756-4646 |
| Popis: | The aim of this study is to research whether stearidonic acid (SDA) has insulinotropic effect and the mechanisms. MIN6 pancreatic β-cells were treated with SDA and effects were analyzed. Protein and mRNA expressions of G protein-coupled receptor 40 (GPR40) increased after 24-h SDA treatment, whereas there was no statistical difference of cellular SDA composition between SDA and control groups. The cytosolic Ca2+ increased in 15 s after treatment, but increment was inhibited when MIN6 cells were preincubated with U-73122 (phospholipase C inhibitor) or BAPTA-AM (Ca2+ chelator). The glucose-stimulated insulin secretion (GSIS) increased after treatment, and GSIS was also inhibited by SDA plus U-73122 or BAPTA-AM. The presence of SDA antagonized palmitic acid-mediated MIN6 cells death. These findings indicate for the first time that SDA can promote GSIS in pancreatic β-cells via stimulation of GPR40-related signaling pathway (direct effect), and via antagonistic effect on long chain saturated fatty acid-mediated impairment (indirect effect). |
| Databáze: | OpenAIRE |
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