Intestinal luminal putrescine is produced by collective biosynthetic pathways of the commensal microbiome
| Autor: | Atsuo Nakamura, Takushi Ooga, Mitsuharu Matsumoto |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Male Arginine Colon Administration Oral Biology Microbiology 03 medical and health sciences chemistry.chemical_compound Feces 0302 clinical medicine Metabolomics Extracellular Putrescine Polyamines stable isotope Animals Microbiome Bacteria arginine degradation Biogenic Polyamines Gastroenterology metabolomics Intestinal microbiome Biosynthetic Pathways Gastrointestinal Microbiome Rats Spermidine Intestines Metabolic pathway 030104 developmental biology Infectious Diseases chemistry Biochemistry Intestinal Microbiome Research Paper/Report 030211 gastroenterology & hepatology |
| Zdroj: | Gut Microbes |
| ISSN: | 1949-0984 |
| Popis: | The intestinal microbiome produces various metabolites that may harm or benefit the host. However, the production pathways of these metabolites have not been well characterised. The polyamines putrescine and spermidine required for physiological process are also produced by intestinal microbiome. The production and release of these polyamines by microbiome are poorly understood, though we have confirmed that intestinal bacteria produced putrescine from arginine. In this study, we characterised polyamine synthesis by analysing the collective metabolic functions of the intestinal microbiome. In particular, we analysed polyamines and their intermediates in faecal cultures, as well as the colonic contents of rats injected with isotope-labelled arginine through a colon catheter, using mass spectrometry. Isotope-labelled putrescine was detected in faecal cultures and colonic contents of rats injected with isotope-labelled arginine. Putrescine is produced through multiple pathways, and its extracellular intermediates are exchanged between bacterial species. Additionally, we demonstrated that the collective metabolic pathway depends on a complex exchange of metabolites released into the colonic lumen. This study demonstrates the existence of putrescine biosynthetic pathways based on the collective metabolic functions of the intestinal microbial community. Our findings provide knowledge to manipulate the levels of intestinal microbial products, including polyamines, that may modulate host health. |
| Databáze: | OpenAIRE |
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