Viability of Glioblastoma Cells and Fibroblasts in the Presence of Imidazole-Containing Compounds
Autor: | Elisabeth Christiane Seidel, Claudia Birkemeyer, Rainer Baran-Schmidt, Jürgen Meixensberger, Henry Oppermann, Frank Gaunitz |
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Rok vydání: | 2022 |
Předmět: |
Alanine
Carnosine Organic Chemistry Imidazoles General Medicine Fibroblasts Catalysis Computer Science Applications Inorganic Chemistry beta-Alanine carnosine glioblastoma fibroblasts imidazole-containing compounds cell viability high-performance liquid chromatography coupled to mass spectrometry Humans Histidine Physical and Theoretical Chemistry Glioblastoma Molecular Biology Spectroscopy Histamine |
Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 10; Pages: 5834 |
ISSN: | 1422-0067 |
DOI: | 10.3390/ijms23105834 |
Popis: | The naturally occurring dipeptide carnosine (β-alanyl-L-histidine) specifically attenuates tumor growth. Here, we ask whether other small imidazole-containing compounds also affect the viability of tumor cells without affecting non-malignant cells and whether the formation of histamine is involved. Patient-derived fibroblasts and glioblastoma cells were treated with carnosine, L-alanyl-L-histidine (LA-LH), β-alanyl-L-alanine, L-histidine, histamine, imidazole, β-alanine, and L-alanine. Cell viability was assessed by cell-based assays and microscopy. The intracellular release of L-histidine and formation of histamine was investigated by high-performance liquid chromatography coupled to mass spectrometry. Carnosine and LA-LH inhibited tumor cell growth with minor effects on fibroblasts, and L-histidine, histamine, and imidazole affected viability in both cell types. Compounds without the imidazole moiety did not diminish viability. In the presence of LA-LH but not in the presence of carnosine, a significant rise in intracellular amounts of histidine was detected in all cells. The formation of histamine was not detectable in the presence of carnosine, LA-LH, or histidine. In conclusion, the imidazole moiety of carnosine contributes to its anti-neoplastic effect, which is also seen in the presence of histidine and LA-LH. Despite the fact that histamine has a strong effect on cell viability, the formation of histamine is not responsible for the effects on the cell viability of carnosine, LA-LH, and histidine. |
Databáze: | OpenAIRE |
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