Reanimation of Myocardial Cells preserved in the Frozen State

Autor:	Freud Ge, Krijnen Hw, Offerijn Fg
Rok vydání:	1969
Předmět:	Cell type Multidisciplinary Lysis Chemistry Myocardium Culture Media Andrology chemistry.chemical_compound Culture Techniques Freezing Glycerol Regeneration Dimethyl Sulfoxide Trypsin Tissue Preservation
Zdroj:	Nature. 222:1174-1175
ISSN:	1476-4687 0028-0836
DOI:	10.1038/2221174a0
Popis:	AFTER the discovery in 1949 of the protective action of glycerol against damage arising from freezing and thawing of spermatozoa1, many investigators have succeeded in freezing different cell types, for example, erythrocytes2,3 and lymphocytes, and cultured tissues with a considerable degree of survival. For certain cell types dimethyl sulphoxide (DMSO) acts as a better cryoprotective substance than glycerol4,5. It has not yet been possible, however, to obtain satisfactory results in the low-temperature preservation of myocardial cells. In a recent publication6 it was stated that 80 to 90 per cent of the myocardial cells in suspension were lysed during freezing; only 5 to 20 per cent of the surviving cells manifested spontaneous beating. We now describe a method for preserving myocardial cells at − 196° C, with a high degree of recovery after thawing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce70c636e2929b751cba48bfd730b7a2 https://doi.org/10.1038/2221174a0 Zobrazit plný text záznamu