The Effects of Variation of Cryopreservation Protocols on the Immunogenicity of AIIogeneic Skin Grafts
| Autor: | John N. Kearney, Eileen Ingham, Gowland G, Matthews Jb |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Glycerol
Time Factors Langerhans cell Ratón Lymphocyte In Vitro Techniques Biology General Biochemistry Genetics and Molecular Biology Cryopreservation Andrology Mice chemistry.chemical_compound Full thickness skin medicine Animals Transplantation Homologous Dimethyl Sulfoxide Skin Mice Inbred BALB C Mice Inbred C3H integumentary system Immunogenicity Graft Survival Significant difference Skin Transplantation General Medicine Cold Temperature medicine.anatomical_structure chemistry Evaluation Studies as Topic Immunology Mice Inbred CBA Female Tissue Preservation Lymphocyte Culture Test Mixed General Agricultural and Biological Sciences |
| Zdroj: | Cryobiology. 30:443-458 |
| ISSN: | 0011-2240 |
| Popis: | The use of allografts to effect wound closure on full thickness skin injuries is limited by unpredictable graft rejection times. If the period of graft take could be extended, the use of allografts would reduce the morbidity and mortality associated with these wounds. This study evaluated the effects of variation in the cryopreservation protocol on the viability and immunogenicity of skin using a murine model system. lmmunogenicity was assessed by the stimulatory activity of C3H (H-2 K ) skin-derived epidermal cells (EC) in primary one-way EC/lymphocyte reactions with BALB/c (H-2 d ) and CBA (H-2 K ) responder lymphocytes. Viability was determined by measuring tetrazolium reductase activity. The following cryopreservation protocols were assessed: freezing at 1, 30, 64, and > 100°C/min in 10 and 15% (v/v) Me 2 SO and freezing at 30°C/min in 5 to 20% (v/v) Me 2 SO or glycerol. A cryopreservation protocol of 30°C/min in 15% (v/v) Me 2 SO proved optimal for murine skin allograft storage and immunomodulation. The viability of skin treated by this protocol was maintained (78% of fresh skin viability, no significant difference analysis of variance). The stimulatory capacity of treated EC for H-2 K and H-2 d lymphocytes was 5 ± 4 and 5 ± 9% (±95% confidence limits) of fresh EC (100%) activity. Langerhans cell numbers in epidermal sheets and EC suspensions did not correlate with the stimulatory capacity of fresh and treated EC for allogeneic Iymphocytes. A functional impairment of Langerhans cell immunostimulatory capacity was implied. Thus it may be feasible to provide a single stage storage and immunomodulation treatment for allogeneic skin. |
| Databáze: | OpenAIRE |
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