Humanized Chimeric Mouse Models to Study Human Neural Development and Pathogenesis of Brain Diseases
| Autor: | Camille Thiberge, Maria Llach Pou, Aline Vitrac, Uwe Maskos, Isabelle Cloëz-Tayarani |
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| Přispěvatelé: | Neurobiologie intégrative des Systèmes cholinergiques / Integrative Neurobiology of Cholinergic Systems (NISC), Institut Pasteur [Paris] (IP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Collège Doctoral, Sorbonne Université (SU), Génétique humaine et fonctions cognitives - Human Genetics and Cognitive Functions (GHFC (UMR_3571 / U-Pasteur_1)), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), The 'Fondation Recherche Alzheimer' provides the funding for CT’s PhD. The Pasteur-Paris University (PPU) international doctoral program provides the funding for MLP’s PhD. The French Ministry of Education and the LabEx BioPsy provided the funding for AV’s PhD. Other funding for this study were provided by grants from the French National Research Agency ANR (ANR-17-NEU3-0004, IPS BRAIN), from ERA-NET NEURON (iPSC & Brain), and from the 'France Alzheimer' foundation. We gratefully acknowledge the UtechS Photonic Bioimaging (Imagopole), C2RT, Institut Pasteur, supported by the French National Research Agency (France BioImaging, ANR-10-INSB-04, Investments for the future)., The authors are thankful to Dr. Alexandra Benchoua who performed the initial iPSC reprogramming, ANR-17-NEU3-0004,iPS_BRAIN,A functional dissection of human nicotinic receptor polymorphisms linked to addiction and schizophrenia(2017), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Translational Research Methods in Neurodevelopmental Disorders Translational Research Methods in Neurodevelopmental Disorders, 185, Springer US, pp.135-158, 2022, Neuromethods, 978-1-0716-2569-9. ⟨10.1007/978-1-0716-2569-9_8⟩ Neuromethods ISBN: 9781071625682 |
| DOI: | 10.1007/978-1-0716-2569-9_8⟩ |
| Popis: | International audience; The development of humanized neural chimeric mouse models consists in grafting human neuronal progenitor cells (NPC) or differentiated neurons derived from induced pluripotent stem cells (iPSC) into the mouse brain at different timepoints. As the main features of cortical networks and their alterations during brain development cannot be reproduced in vitro, these in vivo models have been used to investigate the mechanisms by which reprogrammed human neurons and non-neuronal cells integrate and migrate into the mouse brain at early stages of brain development and display functional activities several months after their grafting. Here, we describe the neonatal grafting technique of human NPC which we use in our laboratory. We also present a new method based on the grafting of human NPC into the brain of mouse embryos, in utero. A third method consists in the stereotaxic grafting of human NPC into selected brain regions of adult mice. The iPSC technology combined with the use of chimeric mouse models offers numerous possibilities to study human neural development within wellcontrolled and defined temporal windows and to model neuropathological disorders. |
| Databáze: | OpenAIRE |
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