High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues
| Autor: | Kaitlyn Sadtler, Ravi Lokwani |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
General Immunology and Microbiology
Cluster of differentiation Biocompatibility medicine.diagnostic_test Staining and Labeling General Chemical Engineering General Neuroscience Cell Immunity Prostheses and Implants Biology Flow Cytometry General Biochemistry Genetics and Molecular Biology Cell biology Staining Flow cytometry medicine.anatomical_structure Immune system In vivo medicine Implant Biomarkers |
| Zdroj: | Journal of visualized experiments : JoVE. (175) |
| ISSN: | 1940-087X |
| Popis: | The success of implanting laboratory-grown tissue or a medical device in an individual is subject to the immune response of the recipient host. Considering an implant as a foreign body, a hostile and dysregulated immune response may result in the rejection of the implant, while a regulated response and regaining of homeostasis can lead to its acceptance. Analyzing the microenvironments of implants dissected out under in vivo or ex vivo settings can help in understanding the pattern of immune response, which can ultimately help in developing new generations of biomaterials. Flow cytometry is a well-known technique for characterizing immune cells and their subsets based on their cell surface markers. This review describes a protocol based on manual dicing, enzymatic digestion, and filtration through a cell strainer for the isolation of uniform cell suspensions from dissected implant tissue. Further, a multicolor flow cytometry staining protocol has been explained, along with steps for initial cytometer settings to characterize and quantify these isolated cells by flow cytometry. |
| Databáze: | OpenAIRE |
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