Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential
| Autor: | A. V. Lisov, Andrey M. Shadrin, Maxim Nagornykh, M. V. Zakharova, Nataliy G. Vinokurova, O. V. Belova, Alexander S. Solonin, Alexey A. Leontievsky, Zhanna I. Andreeva-Kovalevskaya, Zhanna I. Budarina, Alexey Nagel, Zoya A. Lisova |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Xylanases Biophysics Xylose Biology medicine.disease_cause Cellulomonas flavigena 01 natural sciences Applied Microbiology and Biotechnology Saccharification of cereals 03 medical and health sciences chemistry.chemical_compound Hydrolysis Xylooligosaccharides 010608 biotechnology medicine Xylobiose Neutral ph Escherichia coli chemistry.chemical_classification food and beverages Xylan 030104 developmental biology Enzyme Biochemistry chemistry Original Article |
| Zdroj: | AMB Express |
| ISSN: | 2191-0855 |
| Popis: | Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes were more stable at alkaline pH values. CFXyl1 and CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, and xylose, which is typical for GH10. CFXyl3 (GH11) and CFXyl4 (GH10) formed the same xylooligosaccharides, but xylose was formed in small amounts. The xylanases made efficient saccharification of rye, wheat and oat, common components of animal feed, which indicates their high biotechnological potential. |
| Databáze: | OpenAIRE |
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