Effects of administering different vehicles via single intratracheal instillation on responses in the lung and pleural cavity of Crl:CD(SD) rats
| Autor: | Teruaki Hagiwara, Kazunari Uda, Mayumi Kawabe, Taiki Sugiyama, Yuko Doi, Yukinori Mera, Norio Imai, Mai Morioka, Hitomi Higuchi, Takamasa Numano, Seiko Tamano |
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| Rok vydání: | 2020 |
| Předmět: |
040301 veterinary sciences
Intratracheal instillation medicine.medical_treatment Pharmacology Toxicology Pathology and Forensic Medicine 0403 veterinary science 03 medical and health sciences 0302 clinical medicine medicine Diffuse alveolar damage Saline lung toxicity Lung business.industry intratracheal instillation 04 agricultural and veterinary sciences respiratory system Pleural cavity Eosinophil medicine.disease Cell counting medicine.anatomical_structure broncho-alveolar lavage fluid 030220 oncology & carcinogenesis Original Article business Infiltration (medical) |
| Zdroj: | Journal of Toxicologic Pathology |
| ISSN: | 1881-915X 0914-9198 |
| DOI: | 10.1293/tox.2019-0060 |
| Popis: | Intratracheal instillation is the introduction of a substance directly into the trachea. Intratracheal instillation has been used to investigate the lung toxicity of several chemicals and requires the suspension or dissolution of test material in a vehicle for even dispersal throughout the lung. Importantly, the toxicities of vehicles used in intratracheal instillation studies are generally considered to be insignificant. Hence, evaluating the influence of different vehicles on the lung due to intratracheal instillation is crucial. We examined the toxic effects of pure water, saline, phosphate buffered saline (PBS), 0.5% Kolliphor® P188 (KP188), 0.1% Tween 20 in saline, and 1.0% BSA in PBS. These vehicles were administered to male Crl:CD(SD) rats by a single intratracheal instillation. On day 3, broncho-alveolar lavage fluid (BALF) from the right lung was collected and processed for cell counting and biochemical analysis, while the left lung was used for histopathological examination. Accumulation of alveolar macrophages was observed in all vehicle-treated groups but was minimal in the group administered saline, somewhat higher in the groups administered pure water, PBS, 0.1% Tween 20, and 1% BSA, and notably higher in the group administered 0.5% KP188. The results from BALF analysis indicated that intratracheal instillation of 0.5% KP188 also induced alveolar damage. Additionally, administering pure water did not appear to cause tissue damage. Eosinophil infiltration in the interstitial regions was histopathologically observed. Altogether, the results of this study are helpful for the selection of appropriate vehicles for use in intratracheal instillation studies. |
| Databáze: | OpenAIRE |
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