A Novel Cationic Ribonuclease with Antimicrobial Activity from Rana dybowskii
| Autor: | Wei Zhao, Fengyun Tao, Runyu Ma, Meiyin Fan, Qiang Lin |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Staphylococcus aureus
Ranidae RNase P Molecular Sequence Data medicine.disease_cause Biochemistry Anti-Infective Agents Candida albicans Escherichia coli Genetics medicine Animals Amino Acid Sequence Ribonuclease Cloning Molecular Molecular Biology Gene Peptide sequence Phylogeny Ecology Evolution Behavior and Systematics Enzyme Assays biology Ribonuclease Pancreatic General Medicine biology.organism_classification Rana dybowskii Recombinant Proteins Pseudomonas aeruginosa biology.protein Sequence Alignment Function (biology) Antimicrobial Cationic Peptides |
| Zdroj: | Biochemical Genetics. 49:369-384 |
| ISSN: | 1573-4927 0006-2928 |
| DOI: | 10.1007/s10528-010-9414-4 |
| Popis: | A novel ribonuclease (RNase) A superfamily gene (Rdronc) has been cloned from the frog Rana dybowskii. The deduced amino acid sequence shows that it belongs to the ribonuclease A superfamily, with the highest identity, 73%, to Rana pipiens onconase. Adaptive evolution analysis based on maximum likelihood models of codon substitution has been conducted on 10 members of the Rana RNases of subcluster B. Rapid adaptive evolution and multiple positive selection sites have been detected, which indicates that these genes may be evolving under positive selection pressure. Functional assay demonstrates that the recombinant Rdronc protein possesses antimicrobial activity against Gram-negative Escherichia coli and Pseudomonas aeruginosa and weaker antimicrobial activity against Gram-positive Staphylococcus aureus and yeast Candida albicans. Our findings support the hypothesis that ribonuclease A superfamily members may function in host defense of early-diversified vertebrates. |
| Databáze: | OpenAIRE |
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