Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria
| Autor: | D. N. Ostrovsky, A. I. Zinin, M. O. Shleeva, A. S. Kapreliants, G. N. Vostroknutova, A. V. Goncharenko, V. Yu. Artsatbanov |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cell
Mycobacterium smegmatis Mevalonic Acid Oxidative phosphorylation Biology Corynebacterium medicine.disease_cause Biochemistry Microbiology Superoxides medicine Bioorganic chemistry Non-mevalonate pathway chemistry.chemical_classification ATP synthase Terpenes General Medicine Mycobacterium tuberculosis Reactive Nitrogen Species Biosynthetic Pathways Chloroplast Diphosphates Oxidative Stress Enzyme medicine.anatomical_structure chemistry biology.protein Oxidative stress |
| Zdroj: | Biochemistry. Biokhimiia. 77(4) |
| ISSN: | 1608-3040 |
| Popis: | Artificial generation of oxygen superoxide radicals in actively growing cultures of Mycobacterium tuberculosis, Myc. smegmatis, and Corynebacterium ammoniagenes is followed by accumulation in the bacterial cells of substantial amounts of 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (MEcDP) - an intermediate of the non-mevalonate pathway of isoprenoid biosynthesis (MEP) - most possibly due to the interaction of the oxygen radicals with the 4Fe-4S group in the active center and inhibition of the enzyme (E)-4-oxy-3-methylbut-2-enyl diphosphate synthase (IspG). Cadmium ions known to inhibit IspG enzyme in chloroplasts (Rivasseau, C., Seemann, M., Boisson, A. M., Streb, P., Gout, E., Douce, R., Rohmer, M., and Bligny, R. (2009) Plant Cell Environ., 32, 82-92), when added to culture of Myc. smegmatis, substantially increase accumulation of MEcDP induced by oxidative stress with no accumulation of other organic phosphate intermediates in the cell. Corynebacterium ammoniagenes'', well-known for its ability to synthesize large amounts of MEcDP, was also shown to accumulate this unique cyclodiphosphate in actively growing culture when NO at low concentration is artificially generated in the medium. A possible role of the MEP-pathway of isoprenoid biosynthesis and a role of its central intermediate MEcDP in bacterial response to nitrosative and oxidative stress is discussed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|