High Throughput Studies of Cell Migration in 3D Microtissues Fabricated by a Droplet Microfluidic Chip
| Autor: | Jacob A. M. Nuhn, Xiangchen Che, Ian C. Schneider, Long Que |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science lcsh:Mechanical engineering and machinery Nanotechnology 02 engineering and technology cell motility Extracellular matrix 03 medical and health sciences Collagen network microfluidic droplet device lcsh:TJ1-1570 Electrical and Electronic Engineering paracrine Mechanical Engineering Communication Cell migration autocrine 021001 nanoscience & nanotechnology Chip 030104 developmental biology Microfluidic chip Control and Systems Engineering 3D micro-sized tissue Breast cancer cells 0210 nano-technology Biomedical engineering |
| Zdroj: | Micromachines Micromachines, Vol 7, Iss 5, p 84 (2016) |
| ISSN: | 2072-666X |
| Popis: | Arrayed three-dimensional (3D) micro-sized tissues with encapsulated cells (microtissues) have been fabricated by a droplet microfluidic chip. The extracellular matrix (ECM) is a polymerized collagen network. One or multiple breast cancer cells were embedded within the microtissues, which were stored in arrayed microchambers on the same chip without ECM droplet shrinkage over 48 h. The migration trajectory of the cells was recorded by optical microscopy. The migration speed was calculated in the range of 3–6 µm/h. Interestingly, cells in devices filled with a continuous collagen network migrated faster than those where only droplets were arrayed in the chambers. This is likely due to differences in the length scales of the ECM network, as cells embedded in thin collagen slabs also migrate slower than those in thick collagen slabs. In addition to migration, this technical platform can be potentially used to study cancer cell-stromal cell interactions and ECM remodeling in 3D tumor-mimicking environments. |
| Databáze: | OpenAIRE |
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