Functionalization of the surface of electrospun poly(epsilon-caprolactone) mats using zwitterionic poly(carboxybetaine methacrylate) and cell-specific peptide for endothelial progenitor cells capture
| Autor: | Deling Kong, Shufang Wang, Qian Li, Qiang Zhao, Zhihong Wang, Jun Zhang, Wenting Zheng, Lianyong Wang, Suai Zhang |
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| Rok vydání: | 2012 |
| Předmět: |
Blood Platelets
Materials science Cell Survival Surface Properties Aptamer Polyesters Molecular Sequence Data Bioengineering Peptide Methacrylate Umbilical vein Biomaterials Mice Platelet Adhesiveness Polymethacrylic Acids Polymer chemistry Spectroscopy Fourier Transform Infrared Human Umbilical Vein Endothelial Cells Animals Humans Platelet Amino Acid Sequence Progenitor cell chemistry.chemical_classification Photoelectron Spectroscopy Stem Cells Endothelial Cells Water Adhesion Betaine chemistry Mechanics of Materials cardiovascular system Biophysics NIH 3T3 Cells Surface modification Adsorption Rabbits Peptides |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 33(3) |
| ISSN: | 1873-0191 |
| Popis: | A novel approach for vascular grafts to achieve rapid endothelialization is to attract endothelial progenitor cells (EPCs) from peripheral blood onto grafts via EPC-specific antibodies, aptamer, or peptides that specifically bind to EPCs. Inspired by this idea, the electrospun poly(epsilon-caprolactone) (PCL) mats were modified with zwitterionic poly(carboxybetaine methacrylate) (PCBMA) and phage display-selected-EPC-specific peptide, TPSLEQRTVYAK (TPS). We tested the physical and chemical properties, cyto-compatibility, and platelet adhesion of the modified material, and investigated the specificity of the functionalized surface for capturing EPCs. The results indicated that PCL modified with zwitterionic PCBMA and TPS peptide showed improved hydrophilicity without morphology change and damage of the mats. Furthermore, the modified material supported adherence and growth of vascular cells and resisted platelets adhesion. The surfaces also specifically captured EPCs rather than bone marrow mesenchymal stem cells and human umbilical vein endothelial cells. This surface-functionalized PCL graft may offer new opportunities for designing new vascular grafts. |
| Databáze: | OpenAIRE |
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