House dust mite–induced asthma causes oxidative damage and DNA double-strand breaks in the lungs
| Autor: | W.N. Felicia Tan, Tze Khee Chan, W.S. Daniel Tan, W.S. Fred Wong, Xin Yi Loh, Hong Yong Peh, Ian J.J. Tay, Bevin P. Engelward, Na Li |
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| Přispěvatelé: | Massachusetts Institute of Technology. Department of Biological Engineering, Engelward, Bevin, Engelward, Bevin P, Tay, Jun Jie Ian |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Repair DNA repair DNA damage Immunology Respiratory Mucosa medicine.disease_cause Mice 03 medical and health sciences chemistry.chemical_compound medicine Animals Humans Immunology and Allergy DNA Breaks Double-Stranded Antigens Dermatophagoides Lung Cell Death biology Caspase 3 Pyroglyphidae 8-Hydroxy-2'-deoxyguanosine Allergens Molecular biology Asthma 8-Oxoguanine respiratory tract diseases Eosinophils Comet assay Disease Models Animal Oxidative Stress 030104 developmental biology Histone chemistry biology.protein Cytokines Female Inflammation Mediators Reactive Oxygen Species Bronchoalveolar Lavage Fluid DNA Oxidative stress |
| Zdroj: | Prof. Engelward via Howard Silver |
| ISSN: | 0091-6749 |
| DOI: | 10.1016/j.jaci.2016.02.017 |
| Popis: | Background Asthma is related to airway inflammation and oxidative stress. High levels of reactive oxygen and nitrogen species can induce cytotoxic DNA damage. Nevertheless, little is known about the possible role of allergen-induced DNA damage and DNA repair as modulators of asthma-associated pathology. Objective We sought to study DNA damage and DNA damage responses induced by house dust mite (HDM) in vivo and in vitro . Methods We measured DNA double-strand breaks (DSBs), DNA repair proteins, and apoptosis in an HDM-induced allergic asthma model and in lung samples from asthmatic patients. To study DNA repair, we treated mice with the DSB repair inhibitor NU7441. To study the direct DNA-damaging effect of HDM on human bronchial epithelial cells, we exposed BEAS-2B cells to HDM and measured DNA damage and reactive oxygen species levels. Results HDM challenge increased lung levels of oxidative damage to proteins (3-nitrotyrosine), lipids (8-isoprostane), and nucleic acid (8-oxoguanine). Immunohistochemical evidence for HDM-induced DNA DSBs was revealed by increased levels of the DSB marker γ Histone 2AX (H2AX) foci in bronchial epithelium. BEAS-2B cells exposed to HDM showed enhanced DNA damage, as measured by using the comet assay and γH2AX staining. In lung tissue from human patients with asthma, we observed increased levels of DNA repair proteins and apoptosis, as shown by caspase-3 cleavage, caspase-activated DNase levels, and terminal deoxynucleotidyl transferase–mediated dUTP nick end-labeling staining. Notably, NU7441 augmented DNA damage and cytokine production in the bronchial epithelium and apoptosis in the allergic airway, implicating DSBs as an underlying driver of asthma pathophysiology. Conclusion This work calls attention to reactive oxygen and nitrogen species and HDM-induced cytotoxicity and to a potential role for DNA repair as a modulator of asthma-associated pathophysiology. |
| Databáze: | OpenAIRE |
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