Coenzyme Q10 consumption promotes ABCG1-mediated macrophage cholesterol efflux: A randomized, double-blind, placebo-controlled, cross-over study in healthy volunteers
| Autor: | Huihua Li, Tianran Shen, Dongliang Wang, Xilan Tang, Xinwei Jiang, Xiao Yan, Wenhua Ling |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Ubiquinone Biology Pharmacology Young Adult chemistry.chemical_compound Double-Blind Method In vivo Humans Gene Silencing RNA Messenger Cells Cultured ATP Binding Cassette Transporter Subfamily G Member 1 Foam cell Coenzyme Q10 Cross-Over Studies Dose-Response Relationship Drug Activator (genetics) Cholesterol Cholesterol HDL Reverse cholesterol transport Cell Differentiation Cholesterol LDL Healthy Volunteers Gene Expression Regulation ABCG1 chemistry Immunology biology.protein ATP-Binding Cassette Transporters Female lipids (amino acids peptides and proteins) Efflux Foam Cells Signal Transduction Food Science Biotechnology |
| Zdroj: | Molecular Nutrition & Food Research. 59:1725-1734 |
| ISSN: | 1613-4125 |
| Popis: | Scope We have recently shown that coenzyme Q10 (CoQ10) enhances macrophage reverse cholesterol transport via activator protein-1/miRNA-378/ABCG1 signal pathway in vivo and in vitro. Whether CoQ10 exerts similar beneficial effects in human is currently unknown. The present study evaluated the effect of CoQ10 on ABCG1-mediated macrophage cholesterol efflux in 20 healthy volunteers. Methods and results Participants were given 100 mg CoQ10 twice daily or placebo for 1 week with a 1-week washout period. Human monocyte-derived macrophages (MDMs) were differentiated under pooled sera obtained before (preCoQ10) or after CoQ10 (postCoQ10) consumption. The CoQ10-induced inhibition of MDMs foam cell formation was blocked by ABCG1 silencing in postCoQ10 MDMs incubated with postCoQ10 sera compared to preCoQ10 sera. The cholesterol efflux to HDL, and mRNA as well as protein expressions of ATP-binding cassette transporter G1 (ABCG1) were augmented in postCoQ10 MDMs incubated with postCoQ10 sera compared to preCoQ10 sera. The change in the serum CoQ10 concentration positively correlated with cholesterol efflux to HDL and ABCG1 mRNA level in the CoQ10 group. MDMs treated with purified CoQ10 had an enhanced cholesterol efflux to HDL. Conclusion CoQ10 consumption may have an atheroprotective property by inducing ABCG1 expression and enhancing HDL-mediated macrophage cholesterol efflux in healthy individuals. |
| Databáze: | OpenAIRE |
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