Validation of Predicted Virulence Factors in Listeria monocytogenes Identified Using Comparative Genomics

Autor: Reshma Ramachandran, Attila Karsi, Hossam Abdelhamed, Mark L. Lawrence
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Toxins
Volume 11
Issue 9
Toxins, Vol 11, Iss 9, p 508 (2019)
ISSN: 2072-6651
DOI: 10.3390/toxins11090508
Popis: Listeria monocytogenes is an intracellular facultative pathogen that causes listeriosis, a foodborne zoonotic infection. There are differences in the pathogenic potential of L. monocytogenes subtypes and strains. Comparison of the genome sequences among L. monocytogenes pathogenic strains EGD-e and F2365 with nonpathogenic L. innocua CLIP1182 and L. monocytogenes strain HCC23 revealed a set of proteins that were present in pathogenic strains and had no orthologs among the nonpathogenic strains. Among the candidate virulence factors are five proteins: putrescine carbamoyltransferase
InlH/InlC2 family class 1 internalin
phosphotransferase system (PTS) fructose transporter subunit EIIC
putative transketolase
and transcription antiterminator BglG family. To determine if these proteins have a role in adherence and invasion of intestinal epithelial Caco-2 cells and/or contribute to virulence, five mutant strains were constructed. F2365&Delta
inlC2, F2365&Delta
eiic, and F2365&Delta
tkt exhibited a significant (p <
0.05) reduction in adhesion to Caco-2 cells compared to parent F2365 strain. The invasion of F2365&Delta
aguB, F2365&Delta
inlC2, and F2365&Delta
bglG decreased significantly (p <
0.05) compared with the parent strain. Bacterial loads in mouse liver and spleen infected by F2365 was significantly (p <
0.05) higher than it was for F2365&Delta
eiic, F2365&Delta
tkt, and F2365&Delta
bglG strains. This study demonstrates that aguB, inlC2, eiic, tkt, and bglG play a role in L. monocytogenes pathogenicity.
Databáze: OpenAIRE
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