Nicotine in Combination with SARS-CoV-2 Affects Cells Viability, Inflammatory Response and Ultrastructural Integrity

Autor:	Luigi Sansone, Antonio de Iure, Mario Cristina, Manuel Belli, Laura Vitiello, Federica Marcolongo, Alfredo Rosellini, Lisa Macera, Pietro Giorgio Spezia, Carlo Tomino, Stefano Bonassi, Matteo A. Russo, Fabrizio Maggi, Patrizia Russo
Rok vydání:	2022
Předmět:	Lipopolysaccharides Nicotine Cell Survival Interleukin-6 SARS-CoV-2 Tumor Necrosis Factor-alpha pyroptosis Organic Chemistry necroptosis COVID-19 General Medicine cytokines human lung adenocarcinoma A549 cells nicotine poly(I:C) proinflammatory cytokines A549 cells cell survival humans interleukin-6 lipopolysaccharides tumor necrosis factor-alpha Catalysis Computer Science Applications Inorganic Chemistry A549 Cells Cytokines Humans Physical and Theoretical Chemistry Molecular Biology Spectroscopy
Zdroj:	International Journal of Molecular Sciences; Volume 23; Issue 16; Pages: 9488
ISSN:	1422-0067
DOI:	10.3390/ijms23169488
Popis:	The aims of our study are to: (i) investigate the ability of nicotine to modulate the expression level of inflammatory cytokines in A549 cells infected with SARS-CoV-2; (ii) elucidate the ultrastructural features caused by the combination nicotine+SARS-CoV-2; and (iii) demonstrate the mechanism of action. In this study, A549 cells pretreated with nicotine were either exposed to LPS or poly(I:C), or infected with SARS-CoV-2. Treated and untreated cells were analyzed for cytokine production, cytotoxicity, and ultrastructural modifications. Vero E6 cells were used as a positive reference. Cells pretreated with nicotine showed a decrease of IL6 and TNFα in A549 cells induced by LPS or poly(I:C). In contrast, cells exposed to SARS-CoV-2 showed a high increase of IL6, IL8, IL10 and TNFα, high cytopathic effects that were dose- and time-dependent, and profound ultrastructural modifications. These modifications were characterized by membrane ruptures and fragmentation, the swelling of cytosol and mitochondria, the release of cytoplasmic content in extracellular spaces (including osmiophilic granules), the fragmentation of endoplasmic reticulum, and chromatin disorganization. Nicotine increased SARS-CoV-2 cytopathic effects, elevating the levels of inflammatory cytokines, and inducing severe cellular damage, with features resembling pyroptosis and necroptosis. The protective role of nicotine in COVID-19 is definitively ruled out.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce5ce014bcaccfb7d7da17f2b3288ad4 https://doi.org/10.3390/ijms23169488 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.