Drugs/lamellae interface influences the inner structure of double-loaded liposomes for inhaled anti-TB therapy: An in-depth small-angle neutron scattering investigation

Autor: Eleonora Maretti, Fiorella Meneghetti, Eleonora Truzzi, Sarah E. Rogers, Fabio Domenici, Eliana Leo, Angela Capocefalo, Luca Costantino, Carlo Castellano, M. Mori, Valentina Iannuccelli
Rok vydání: 2018
Předmět:
Drug
Materials science
media_common.quotation_subject
Multilamellar liposomes
Antitubercular Agents
02 engineering and technology
Coatings and Films
Biomaterials
03 medical and health sciences
Colloid and Surface Chemistry
Drug Delivery Systems
In vivo
Scattering
Small Angle
Electronic
Isoniazid
Multilamellar liposomes
Drugs-lamellae interactions
Small-angle neutron scattering
Isoniazid
Rifampicin
Optical and Magnetic Materials
Rifampicin
030304 developmental biology
media_common
Drugs-lamellae interactions
Small-angle neutron scattering
Settore CHIM/02 - Chimica Fisica
0303 health sciences
Liposome
Drug Carriers
Bilayer
bacterial infections and mycoses
021001 nanoscience & nanotechnology
Surfaces
Coatings and Films
Electronic
Optical and Magnetic Materials
Surfaces
Drug Liberation
Liposomes
Biophysics
Drug release
Rifampin
0210 nano-technology
Drug carrier
Zdroj: Journal of colloid and interface science. 541
ISSN: 1095-7103
Popis: With the aim of developing new drug carriers for inhalation therapy, we report here an in depth investigation of the structure of multilamellar liposomes loaded with two well-established anti-tubercular (anti-TB) drugs, isoniazid (INH) and rifampicin (RIF), by means of small-angle neutron-scattering (SANS) analysis. Unloaded, single drug-loaded and co-loaded liposomes were prepared using different amounts of drugs and characterized regarding size, encapsulation efficiency and drug release. Detailed information on relevant properties of the investigated host-guest structures, namely the steric bilayer thickness, particle dispersion, number of lamellae and drug localization was studied by SANS. Results showed that RIF-liposomes were less ordered than unloaded liposomes. INH induced a change in the inter-bilayer periodical spacing, while RIF-INH co-loading stabilized the multilamellar liposome architecture, as confirmed by the increment of the drug loading capacity. These findings could be useful for the understanding of in vitro and in vivo behavior of these systems and for the design of new drug carriers, intended for inhaled therapy.
Databáze: OpenAIRE
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