Simultaneous Monitoring of Wireless Electrophysiology and Memory Behavioral Test as a Tool to Study Hippocampal Neurogenesis
| Autor: | Luis Miguel Rodríguez-Serrano, Oscar Galicia-Castillo, Sylvia Ortega-Martínez, Daniel Tirado-Martínez, Erik Bojorges-Valdez, Florencia Mata, Jesús Mata-Luévanos, Alejandro Tapia-de-Jesús, María Elena Chávez-Hernández, Mario Buenrostro-Jáuregui |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Brain activity and meditation Computer science General Chemical Engineering Neurogenesis Short-term memory Electroencephalography Hippocampus General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Memory medicine Animals Rats Wistar 030304 developmental biology 0303 health sciences General Immunology and Microbiology medicine.diagnostic_test Behavior Animal business.industry Long-term memory General Neuroscience Pattern recognition Object (computer science) Brain Waves Electrodes Implanted Rats Task (computing) Electrophysiology Artificial intelligence business Wireless Technology 030217 neurology & neurosurgery Cognitive load |
| Zdroj: | Journal of visualized experiments : JoVE. (162) |
| ISSN: | 1940-087X |
| Popis: | Brainwaves amplitude obtained from electroencephalography (EEG) has been well-recognized as a basis for cognitive capacity, memory, and learning on animals and humans. Adult neurogenesis mechanism is also linked to memory and learning improvement. Traditionally, researchers used to assess learning and memory parameters in rodent models by behavioral tasks. Therefore, the simultaneous monitoring of behavioral changes and EEG is particularly interesting in correlating data between brain activity and task-related behaviors. However, most of the equipment required to perform both studies are either complex, expensive, or uses a wired setup network that hinders the natural animals' movement. In this study, EEG was recorded with a wireless electrophysiology device along with the execution of a novel object recognition task (NORT). The animal's behavior was monitored simultaneously by a video tracking system. Both recordings were analyzed offline by their timestamps which were synched to link EEG signals with the animal's actions. Subjects consist of adult Wistar rats after medium-term environmental enrichment treatment. Six skull screw electrodes were fixed in pairs on both hemispheres over frontal, central, and parietal regions and were referenced to an electrode located posterior of the nasal bone. NORT protocol consists of exposing the animal to two identical objects for 10 min. After 2 h and 24 h, one of the objects was replaced with a novel one. Exploration time for each object was monitored by a behavioral tracking software (BTS) and EEG data recording. The analysis of the EEG synced with behavioral data consists of estimations of alpha and beta relative band power and comparisons between novel object recognition versus familiar object exploration, between three experimental stages. In this manuscript, we have discussed electrodes manufacturing process, epidural electrodes implantation surgery, environmental enrichment protocol, NORT protocol, BTS setup, EEG - BTS coupling for simultaneous monitoring in real-time, and EEG data analysis based on automatic events detection. |
| Databáze: | OpenAIRE |
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