A NovelLeishmania infantumRecombinant Antigen Which Elicits Interleukin 10 Production by Peripheral Blood Mononuclear Cells of Patients with Visceral Leishmaniasis
| Autor: | Xavier Stien, Pierre Marty, Baharia Mograbi, D Rousseau, Bernard Ferrua, J. Kubar, Konstantina Fragaki, Isabelle Suffia |
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| Rok vydání: | 2000 |
| Předmět: |
Immunogen
Molecular Sequence Data Immunology Antibodies Protozoan Antigens Protozoan Microbiology Peripheral blood mononuclear cell Antigen parasitic diseases medicine Animals Amino Acid Sequence Cloning Molecular Leishmania infantum biology Immune Sera Leishmaniasis biology.organism_classification medicine.disease Leishmania Virology Recombinant Proteins Interleukin-10 Infectious Diseases Visceral leishmaniasis Immunoglobulin M Leukocytes Mononuclear biology.protein Leishmaniasis Visceral Female Parasitology Rabbits Fungal and Parasitic Infections |
| Zdroj: | Infection and Immunity. 68:630-636 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | We report here the characterization of a novelLeishmania infantumprotein termed papLe22 (22-kDa potentially aggravating protein ofLeishmania). A positive clone from a cDNA library was identified by serum of a visceral leishmaniasis (VL) patient. Full-length cDNA obtained using rapid amplification of cDNA ends-PCR codes for a 22-kDa protein. InL. infantumpromastigotes an endogenous nuclear protein of 14-kDa electrophoretic mobility was found by using an antiserum prepared against the fusion protein glutathioneS-transferase–papLe22. Its expression was also shown inL. infantumamastigotes and inLeishmania majorandLeishmania guyanensispromastigotes. VL patients' sera showed anti-papLe22 immunoglobulin M (IgM) and IgG reactivities, indicating that a primary response against the leishmanial protein papLe22 accompanied acute VL manifestations. Specific IgG levels were correlated with patients' clinical status. The presence of IgG1, IgG2, and IgG3 subclasses suggested a mixed Th1- and Th2-type response; there was no correlation between subclass reactivity and the disease course. The recombinant papLe22 specifically activated interleukin-10 production by VL patients' peripheral blood mononuclear cells collected at diagnosis and after treatment-induced cure, indicating its contribution to VL pathogenesis and concomitant immunosuppression and its potential role in the reactivation of latent parasites. As a dominant immunogen, papLe22 might be used as a vaccine component, provided that the vaccination protocol directs the response toward the Th1 pattern. |
| Databáze: | OpenAIRE |
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