Characterization of hepatoprotective metabolites from Artemisia annua and Cleome droserifolia using HPLC/PDA/ESI/MS–MS
Autor: | Hesham I. El-Askary, Amira Abdel Motaal, Abdulrhman Alsayari, Ahmed H. El-Khatib, Heba Handoussa, Michael W. Linscheid, Farid A. Badria |
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Rok vydání: | 2019 |
Předmět: |
Antioxidant
medicine.medical_treatment Artemisia annua lcsh:RS1-441 Aspartate transaminase 01 natural sciences High-performance liquid chromatography lcsh:Pharmacy and materia medica HepG-2 medicine MTT assay General Pharmacology Toxicology and Pharmaceutics Herb biology Traditional medicine 010405 organic chemistry Chemistry Traditional Asteraceae biology.organism_classification Hepatoprotective 0104 chemical sciences 010404 medicinal & biomolecular chemistry Alanine transaminase HPLC/PDA/ESI/MS–MS biology.protein Cleomaceae |
Zdroj: | Revista Brasileira de Farmacognosia, Volume: 29, Issue: 2, Pages: 213-220, Published: 27 MAY 2019 Revista Brasileira de Farmacognosia, Vol 29, Iss 2, Pp 213-220 (2019) Revista Brasileira de Farmacognosia v.29 n.2 2019 Revista Brasileira de Farmacognosia Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
ISSN: | 0102-695X |
DOI: | 10.1016/j.bjp.2018.10.001 |
Popis: | The hepatoprotective activities of two traditionally used plants, Cleome droserifolia (Forssk.) Delile, Cleomaceae, and Artemisia annua L., Asteraceae, were recently reported. However, the biologically active metabolites responsible for this activity were not identified. The aqueous extract of C. droserifolia aerial parts, and the polar fraction of A. annua leaves were screened for their antioxidant activities using the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) assay. The in vitro viability of HepG-2 cells treated with CCl4 and the extracts were assessed by MTT assay. The effects of the extracts on the liver enzymes and the total soluble protein in CCl4-intoxicated HepG-2 cells were investigated. An HPLC/PDA/ESI/MS–MS based analysis was carried out for extract of C. droserifolia and polar fraction of A. annua. Both exhibited pronounced free radical scavenging activities (86 and 83%, respectively). Both showed a significant increase in cell viability: 86.43% for the extract of C. droserifolia and 79.32% for polar fraction of A. annua. Only the extract of C. droserifolia (39.6 ± 5.41 and 20.4 ± 6.91 IU/dl, respectively) and polar fraction of A. annua (40.8 ± 2.14 and 24.5 ± 3.11 IU/dl, respectively) restored the levels of liver enzymes (aspartate transaminase and alanine transaminase, respectively) compared to the CCl4 intoxicated group (87.5 ± 4.34 and 34.1 ± 8.12 IU/dl, respectively) and other herbal extracts. More than fifty phenolic secondary metabolites were identified in the extracts under investigation. The significant hepatoprotective activities of both extracts seemed to be strongly connected to their content of hydroxycinnamoyl quinic acids and flavonoids. Keywords: Herb, Traditional, HPLC/PDA/ESI/MS–MS, Hepatoprotective, HepG-2, Antioxidant |
Databáze: | OpenAIRE |
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