Anatomical distribution and daily profile of gper1b gene expression in brain and peripheral structures of goldfish (Carassius auratus)
| Autor: | Marie-M. Gueguen, Ángel Luis Alonso-Gómez, María Jesús Delgado, Esther Isorna, Joel Cano-Nicolau, Olivier Kah, Aída Sánchez-Bretaño |
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| Přispěvatelé: | Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) |
| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Cerebellum Habenular nuclei Time Factors Physiology Photoperiod [SDV]Life Sciences [q-bio] clock gene In situ hybridization Biology Real-Time Polymerase Chain Reaction liver pituitary Midbrain Biological Clocks Physiology (medical) Internal medicine Goldfish medicine Animals Circadian rhythm RNA Messenger Cloning Molecular Intestinal Mucosa intestine Cerebrum Brain Period Circadian Proteins Circadian Rhythm CLOCK Endocrinology medicine.anatomical_structure Gene Expression Regulation nervous system Forebrain circadian system Female in situ hybridization sense organs |
| Zdroj: | Chronobiology International Chronobiology International, 2015, 32 (7), pp.889--902. ⟨10.3109/07420528.2015.1049615⟩ Chronobiology International, Taylor & Francis, 2015, 32 (7), pp.889--902. ⟨10.3109/07420528.2015.1049615⟩ |
| ISSN: | 0742-0528 1525-6073 |
| DOI: | 10.6084/m9.figshare.1569824.v3 |
| Popis: | The functional organization of the circadian system and the location of the main circadian oscillators vary through phylogeny. Present study investigates by in situ hybridization the anatomical location of the clock gene gPer1b in forebrain and midbrain, pituitary, and in two peripheral locations, the anterior intestine and liver, in a teleost fish, the goldfish (Carassius auratus). Moreover, the daily expression profiles of this gene were also studied by quantitative Real Time-PCR. Goldfish were maintained under a 12L–12D photoperiod and fed daily at 2 h after lights were switched on. A wide distribution of gPer1b mRNA in goldfish brain and pituitary was found in telencephalon, some hypothalamic nuclei (including the homologous to mammalian SCN), habenular nucleus, optic tectum, cerebellum and torus longitudinalis. Moreover, gPer1b expression was observed, for the first time in teleosts, in the pituitary, liver and anterior intestine. Day/night differences in gper1b mRNA abundance were found by in situ hybridization, with higher signal at nighttime that correlates with the results obtained by RT-PCR, where a rhythmic gPer1b expression was found in all tissues with acrophases at the end of the night. Amplitudes of gper1b rhythms vary among tissues, being higher in liver and intestine than in the brain, maybe because different cues entrain clocks in these locations. These results support the existence of functional clocks in many central and peripheral locations in goldfish coordinated, ticking at the same time. |
| Databáze: | OpenAIRE |
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