Enhancement of monoclonal antibody yield by hybridoma fed-batch culture, resulting in extended maintenance of viable cell population

Autor:	R.E. Spier, Michael E. Bushell, S. L. Bell, M. F. Scott, John N. Wardell, P. G. Sanders
Rok vydání:	1994
Předmět:	education.field_of_study Cell growth medicine.drug_class business.industry Population Antibody titer Bioengineering Biology Monoclonal antibody Applied Microbiology and Biotechnology Biotechnology Fed-batch culture Cell culture medicine Bioreactor Viability assay Food science business education
Zdroj:	Biotechnology and Bioengineering. 44:1099-1106
ISSN:	1097-0290 0006-3592
DOI:	10.1002/bit.260440911
Popis:	Hybridoma batch cultures were extended using feed formulations based on nutrient consumption measured during different batch culture phases when (a) growth but negligible antibody production was taking place; (b) maximum antibody production rate and declining viable cell growth rate were observed. Strategy (a) was the more successful (2.8-fold compared with 1.8-fold antibody titer increase) and maintained cell viability for longer. Analysis of the effects of omitting individual amino acids yielded results which were consistent with those from the feeding experiment © 1994 John Wiley & Sons, Inc.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce57a18a65d5d6a53cca403b17db21ba https://doi.org/10.1002/bit.260440911 Zobrazit plný text záznamu Plný text