Multiple resistance phenotypes to Lettuce mosaic virus among Arabidopsis thaliana accessions
| Autor: | Frédéric Revers, Thierry Mauduit, Marie-Christine Houvenaghel, Thierry Candresse, Thomas Guiraud, Olivier Le Gall |
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| Přispěvatelé: | Génomique, développement et pouvoir pathogène (GD2P), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA) |
| Předmět: |
0106 biological sciences
virus des vegetaux Physiology Arabidopsis lmv 01 natural sciences Pathosystem Gene Expression Regulation Plant potyvirus virus de la mosaïque de la laitue Arabidopsis thaliana Eukaryotic Initiation Factors ComputingMilieux_MISCELLANEOUS Genes Dominant Genetics 0303 health sciences biology Potyvirus General Medicine Lettuce Lettuce mosaic virus VIROLOGIE Phenotype plante Molecular Sequence Data BIOLOGIE MOLECULAIRE RESISTANCE Genes Plant Cape verde 03 medical and health sciences Mosaic Viruses Genetic variation [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Amino Acid Sequence Plant Diseases 030304 developmental biology Mosaic virus gène arabidopsis thaliana Genetic Variation biology.organism_classification Virology Immunity Innate Plant Leaves Sequence Alignment Agronomy and Crop Science analyse génétique 010606 plant biology & botany |
| Zdroj: | HAL Molecular Plant-Microbe Interactions 7 (16), 608-616. (2003) Molecular Plant-Microbe Interactions Molecular Plant-Microbe Interactions, American Phytopathological Society, 2003, 16 (7), pp.608-616 Scopus-Elsevier |
| ISSN: | 0894-0282 |
| Popis: | With the aim to characterize plant and viral factors involved in the molecular interactions between plants and potyviruses, a Lettuce mosaic virus (LMV)-Arabidopsis thaliana pathosystem was developed. Screening of Arabi-dopsis accessions with LMV isolates indicated the existence of a large variability in the outcome of the interaction, allowing the classification of Arabidopsis accessions into seven susceptibility groups. Using a reverse genetic approach, the genome-linked protein of LMV, a multifunc-tional protein shown to be involved in viral genome amplification and movement of potyviruses, was established as the viral determinant responsible for the ability to overcome the resistance of the Niederzenz accession to LMV-0. Preliminary genetic analyses from F2 and recombinant inbred lines available between susceptible and resistant Arabidopsis accessions revealed the existence of at least three resistance phenotypes to LMV with different genetic bases. One dominant resistance gene, designated LLM1, involved in blocking the replication or cell-to-cell movement of the LMV-0 isolate in the Columbia accession, was mapped to chromosome I and shown to be linked to the marker nga280. At the same time, genetic analyses of segregating F2 populations were consistent with the restriction of the systemic movement of the LMV-AF199 isolate in Columbia being controlled by two dominant genes and with the complete resistance to all tested LMV isolates of the Cape Verde islands (Cvi) accession being conferred by a single recessive resistance gene. Sequencing of the eu-karyotic translation initiation factor 4E genes from the different LMV-resistant Arabidopsis accessions showed that these factors are not directly involved in the characterized resistance phenotypes. |
| Databáze: | OpenAIRE |
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