Blocking ELISA using recombinant NcSRS2 protein for diagnosing bovine neosporosis
| Autor: | Leonardo Garcia Monte, Sibele Borsuk, Bruno Moisés de Matos, Diene de Borba Pacheco, Francine Alves Sinnott, Renato Andreotti, Thaís Farias Collares, Cláudia Pinho Hartleben |
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| Rok vydání: | 2014 |
| Předmět: |
High-throughput screening
Protozoan Proteins Cattle Diseases Antigens Protozoan Enzyme-Linked Immunosorbent Assay Applied Microbiology and Biotechnology Microbiology Sensitivity and Specificity Antibodies law.invention Serology law parasitic diseases Animals Antibodies Blocking Direct fluorescent antibody biology Coccidiosis fungi Neospora Reproducibility of Results General Medicine bacterial infections and mycoses biology.organism_classification Virology Neospora caninum Polyclonal antibodies Antigens Surface Recombinant DNA biology.protein Cattle Antibody |
| Zdroj: | Current microbiology. 70(3) |
| ISSN: | 1432-0991 |
| Popis: | Neospora caninum is the etiologic agent of neosporosis, which leads to economic impacts on cattle industry. The reference method for serodiagnosis of neosporosis is the indirect fluorescent antibody test (IFAT). However, IFAT is laborious, expensive, and is not practicable in high throughput screening. In order to facilitate the serological diagnosis of neosporosis, we developed a blocking enzyme-linked immunosorbent assay (b-ELISA) based on NcSRS2 recombinant protein (rNcSRS2) and polyclonal antibodies against rNcSRS2 (b-ELISA/rNcSRS2). Compared to IFAT, b-ELISA/rNcSRS2 showed 93.7 % accuracy (98.7 % sensitivity and 88.7 % specificity), suggesting its potential as diagnostic assay to detect N. caninum antibodies in cattle sera. |
| Databáze: | OpenAIRE |
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