Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector

Autor: Heidi Yuan, Qin Xu, Dah-Eun Jeong, Ramsey Najm, Maureen E. Balestra, Zachary A. Miller, Yadong Huang, Mary J. Malloy, Bruce L. Miller, Gang Li, Chengzhong Wang, David Walker, Seo Yeon Yoon
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Apolipoprotein E
Aging
Apolipoprotein E4
Apolipoprotein E3
Neurodegenerative
Alzheimer's Disease
Medical and Health Sciences
Genome editing
2.1 Biological and endogenous factors
Protein Isoforms
Aetiology
Phosphorylation
GABAergic Neurons
Induced pluripotent stem cell
Cells
Cultured

Neurons
Gene Editing
Cultured
Homozygote
General Medicine
Phenotype
Small molecule
Cell biology
Neurological
lipids (amino acids
peptides
and proteins)

Animal studies
Cells
Induced Pluripotent Stem Cells
Immunology
tau Proteins
Biology
General Biochemistry
Genetics and Molecular Biology

Article
Cell Line
Small Molecule Libraries
03 medical and health sciences
Alzheimer Disease
mental disorders
Genetics
Acquired Cognitive Impairment
Humans
Amyloid beta-Peptides
Neurosciences
Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD)
Stem Cell Research
Brain Disorders
030104 developmental biology
Cell culture
Nerve Degeneration
APOE Gene Product
Dementia
human activities
Zdroj: Wang, C; Najm, R; Xu, Q; Jeong, D-E; Walker, D; Balestra, ME; et al.(2018). Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector. NATURE MEDICINE, 24(5), 647-+. doi: 10.1038/s41591-018-0004-z. UCSF: Retrieved from: http://www.escholarship.org/uc/item/796161bm
Nature medicine, vol 24, iss 5
Nature medicine
DOI: 10.1038/s41591-018-0004-z.
Popis: Efforts to develop drugs for Alzheimer's disease (AD) have shown promise in animal studies, only to fail in human trials, suggesting a pressing need to study AD in human model systems. Using human neurons derived from induced pluripotent stem cells that expressed apolipoprotein E4 (ApoE4), a variant of the APOE gene product and the major genetic risk factor for AD, we demonstrated that ApoE4-expressing neurons had higher levels of tau phosphorylation, unrelated to their increased production of amyloid-β (Aβ) peptides, and that they displayed GABAergic neuron degeneration. ApoE4 increased Aβ production in human, but not in mouse, neurons. Converting ApoE4 to ApoE3 by gene editing rescued these phenotypes, indicating the specific effects of ApoE4. Neurons that lacked APOE behaved similarly to those expressing ApoE3, and the introduction of ApoE4 expression recapitulated the pathological phenotypes, suggesting a gain of toxic effects from ApoE4. Treatment of ApoE4-expressing neurons with a small-molecule structure corrector ameliorated the detrimental effects, thus showing that correcting the pathogenic conformation of ApoE4 is a viable therapeutic approach for ApoE4-related AD.
Databáze: OpenAIRE