A Porcine Model of Acute Lung Injury by Instillation of Gastric Fluid
| Autor: | Veerle Mertens, Robin Vos, Dirk Van Raemdonck, Shana Wauters, Geert Verleden, Eric Verbeken, Caroline Marie F Meers, Bart M. Vanaudenaerde, Stéphanie De Vleeschauwer, Walter De Wever |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Pulmonary Circulation medicine.medical_specialty Pathology Swine medicine.medical_treatment Acute Lung Injury Hemodynamics Lung injury Pulmonary compliance Bronchoalveolar Lavage Gastroenterology Internal medicine medicine Animals Lung transplantation Saline Gastric Juice medicine.diagnostic_test Pulmonary Gas Exchange business.industry Airway Resistance Respiratory Aspiration Pneumonia Oxygenation respiratory system Tissue Donors Specific Pathogen-Free Organisms respiratory tract diseases Radiography Transplantation Disease Models Animal Bronchoalveolar lavage Surgery business Bronchoalveolar Lavage Fluid Lung Transplantation |
| Zdroj: | Journal of Surgical Research. 166:e195-e204 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2010.10.015 |
| Popis: | About 15% of donor lungs are declined because of aspiration contributing to current organ shortage. The aim was to develop a porcine lung injury model by gastric juice (GJ) instillation to study different pretransplant treatment strategies.Pigs (n = 6/group) were anesthetized and monitored. At T0 bronchoalveolar lavage (BAL) was performed followed by instillation of 4 mL/kg GJ or saline solution (SAL). Hemodynamics, aerodynamics and oxygenation were recorded for two hours. Serum samples were collected. At T120 a second BAL was performed. CT scans of explanted, inflated lungs were taken, tissue samples were collected and wet/dry weight ratio (W/D) was calculated. Pepsin and bile acids were measured in BAL. IL-8, CRP and MMP-9 were measured in serum and in BAL.Oxygenation and lung compliance was lower in [GJ] versus [SAL] (P0.01 and P0.001, respectively). More consolidation areas were noticed on CT in GJ versus SAL (P0.01). Hemorrhage, edema and neutrophil inflammation were seen on histology in [GJ] (P0.01, P0.001, P0.001, respectively). BAL neutrophils, pepsin, bile acids, and IL-8 (P0.05) increased in [GJ]. W/D was higher in [GJ] versus SAL (P0.001).Instillation of GJ in pig lungs caused acute lung injury with impaired oxygenation and increased inflammation in BAL, on histology, and on imaging. This model holds promise to assess the efficacy of a broad range of treatment strategies including ex vivo lung perfusion (EVLP). |
| Databáze: | OpenAIRE |
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