Transgenic Tobacco Plants Expressing the Drosophila Polycomb (Pc) Chromodomain Show Developmental Alterations: Possible Role of Pc Chromodomain Proteins in Chromatin-Mediated Gene Regulation in Plants
| Autor: | Bénédicte Charrier, Claire Scollan, Peter Meyer, Richard Ingram |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Heterochromatin
Recombinant Fusion Proteins Green Fluorescent Proteins Molecular Sequence Data Polycomb-Group Proteins Plant Science Biology Polymerase Chain Reaction Chromodomain Gene Expression Regulation Plant Tobacco DNA Primers Plant Proteins SUV39H1 Cell Nucleus Genetics Regulation of gene expression Base Sequence Plant Stems fungi food and beverages Cell Biology Plants Genetically Modified Chromatin Recombinant Proteins Plant Leaves Repressor Proteins Luminescent Proteins Plants Toxic Homeobox Heterochromatin protein 1 Homeotic gene Research Article |
| Zdroj: | The Plant Cell. 11:1047-1060 |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.11.6.1047 |
| Popis: | The chromodomain of the Drosophila Polycomb (Pc) protein has been introduced into tobacco nuclei to determine its location in the nucleus and its effect on plant development. Pc is a repressor of homeotic Drosophila genes that shares a well-conserved, although not identical, chromodomain with a structural heterochromatin component, Heterochromatin Protein 1. The chromodomains might therefore play a common role in chromatin repression. An analysis of transgenic plants expressing the Pc chromodomain, which was linked to the green fluorescent protein, suggested that the Pc chromodomain has distinct target regions in the plant genome. Transgenic plants expressing the Pc chromodomain had phenotypic abnormalities in their leaves and flowers, indicating a disruption in development. In axillary shoot buds of plants displaying altered leaf phenotypes, enhanced expression of a homeodomain gene, which is downregulated in wild-type leaves, was found. In Drosophila, Pc has been shown to possess distinct chromosome binding activity and to be involved in the regulation of development-specific genes. Our results support the assumptions that the heterologous chromodomain affects related functions in Drosophila and in plants, and that chromatin modification mechanisms are involved in the regulation of certain plant genes, in a manner similar to chromatin-mediated gene regulation in Drosophila. |
| Databáze: | OpenAIRE |
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