Enrichment of immunoregulatory proteins in the biomolecular corona of nanoparticles within human respiratory tract lining fluid
| Autor: | Matthew Arno, Jonny Eriksson, Elif Melis Bicer, Ben Forbes, Annelie F. Behndig, Anna Babin Morgan, Dave Hassall, Katarina Edwards, Graham Somers, Paul E Pfeffer, Kenneth A. Dawson, Lea Ann Dailey, Abhinav Kumar, Marco P. Monopoli, Steven Lynham, Ian Mudway, Anders Blomberg |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Proteomics 0301 basic medicine endocrine system Materials science Respiratory System Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Nanoparticle Bioengineering Protein Corona Nanotechnology 02 engineering and technology 03 medical and health sciences Drug Delivery Systems medicine Aspartic Acid Endopeptidases Humans General Materials Science Innate immune system Pulmonary Surfactant-Associated Protein A Complement C1q Surfactant protein D Complement C3 Silicon Dioxide 021001 nanoscience & nanotechnology Body Fluids Surfactant protein A 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Drug delivery Biophysics Nanoparticles Molecular Medicine Female 0210 nano-technology Respiratory tract |
| Zdroj: | Nanomedicine: Nanotechnology, Biology and Medicine. 12:1033-1043 |
| ISSN: | 1549-9634 |
| Popis: | When inhaled nanoparticles deposit in the lungs, they transit through respiratory tract lining fluid (RTLF) acquiring a biomolecular corona reflecting the interaction of the RTLF with the nanomaterial surface. Label-free snapshot proteomics was used to generate semi-quantitative profiles of corona proteins formed around silica (SiO2) and poly(vinyl) acetate (PVAc) nanoparticles in RTLF, the latter employed as an archetype drug delivery vehicle. The evolved PVAc corona was significantly enriched compared to that observed on SiO2 nanoparticles (698 vs. 429 proteins identified); however both coronas contained a substantial contribution from innate immunity proteins, including surfactant protein A, napsin A and complement (C1q and C3) proteins. Functional protein classification supports the hypothesis that corona formation in RTLF constitutes opsonisation, preparing particles for phagocytosis and clearance from the lungs. These data highlight how an understanding of the evolved corona is necessary for the design of inhaled nanomedicines with acceptable safety and tailored clearance profiles.Inhaled nanoparticles often acquire a layer of protein corona while they go through the respiratory tract. Here, the authors investigated the identity of these proteins. The proper identification would improve the understanding of the use of inhaled nanoparticles in future therapeutics. |
| Databáze: | OpenAIRE |
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