Identification of colon-tumor-associated antigens by T-cell lines derived from tumor-infiltrating lymphocytes and peripheral-blood lymphocytes from patients immunized with an autologous tumor-cell/bacillus calmette-Guérin vaccine
| Autor: | Nicholas Pomato, Henk A. J. M. Hubers, Janet H. Ransom, Michael G. Hanna, Linette M. Keynton, Barbara A. Pelle |
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| Rok vydání: | 1993 |
| Předmět: |
Cancer Research
medicine.medical_treatment Mice Nude Gene Rearrangement T-Lymphocyte Major histocompatibility complex Bacillus Calmette Guerin vaccine Mice Lymphocytes Tumor-Infiltrating Antigen Antigens Neoplasm HLA Antigens Tumor Cells Cultured medicine Animals Humans Lymphocytes Cell Line Transformed Glycoproteins biology Tumor-infiltrating lymphocytes Antibodies Monoclonal Immunotherapy T lymphocyte Autologous tumor cell Carcinoembryonic Antigen Oncology Colonic Neoplasms Immunology BCG Vaccine biology.protein Immunization BCG vaccine |
| Zdroj: | International Journal of Cancer. 54:734-740 |
| ISSN: | 1097-0215 0020-7136 |
| DOI: | 10.1002/ijc.2910540505 |
| Popis: | Tumor immunity developing as a response to an autologous colon-tumor/bacillus Calmette-Guérin (BCG) vaccine appears to be associated with induction of CD4+ helper T cells, implied by the observation that vaccine efficacy is associated with major histocompatibility complex class-II molecule expression on the vaccine tumor cells. Therefore, in an attempt to identify colon-tumor-associated antigens responsible for conferring immunity, we examined and compared the proliferative responses of peripheral-blood lymphocytes (PBL) from patients immunized with the autologous tumor/BCG vaccine to T-cell lines cloned expanded from colon-tumor-infiltrating lymphocytes to 5 antigens isolated on the basis of their reactivity by colon-tumor-reactive human monoclonal antibodies. Enzymatically dissociated colon tumors provided a source for establishment of cloned T-cell lines, tumor cell lines propagated in vitro or in vivo as nude-mouse xenografts and EBV-transformed B-cell lines used as antigen-presenting cells. Of 104 different T-cell lines tested, only 3 proliferated in response to CTAA 28A32-46K, and I to the CTAA28A32-32K antigen. In contrast, PBL from 64% of patients immunized with the autologous colon-tumor/BCG vaccine responded to the CTAA 28A32-32K antigen. This antigen is related to a family of calcium- and phospholipid-binding placental proteins termed annexins. Since proliferative responses developed to this antigen after vaccination in 64% of individuals, this antigen may be an important common colon-tumor-associated rejection antigen. |
| Databáze: | OpenAIRE |
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