Protective effects of dietary omega-3 fatty acid supplementation on organophosphate poisoning

Autor: Ozge Darakci, S. Sırrı Bilge, Bahattin Avci, Omer Alici, Engin Ciftcioglu, Ayhan Bozkurt, Türker Yardan, Turkhan Baratzada, Gokhan Arslan
Přispěvatelé: [Avci, Bahattin -- Baratzada, Turkhan] Ondokuz Mayis Univ, Dept Biochem, Fac Med, Samsun, Turkey -- [Bilge, S. Sirri] Ondokuz Mayis Univ, Dept Pharmacol, Fac Med, Samsun, Turkey -- [Arslan, Gokhan] Cumhuriyet Univ, Dept Physiol, Fac Med, Sivas, Turkey -- [Alici, Omer] Gaziosmanpasa Univ, Dept Pathol, Fac Med, Tokat, Turkey -- [Darakci, Ozge -- Bozkurt, Ayhan] Ondokuz Mayis Univ, Dept Physiol, Fac Med, Samsun, Turkey -- [Ciftcioglu, Engin] Ondokuz Mayis Univ, Dept Anat, Fac Med, Samsun, Turkey -- [Yardan, Turker] Ondokuz Mayis Univ, Dept Emergency Med, Fac Med, Samsun, Turkey, Arslan, Gokhan -- 0000-0003-4186-2478, Bilge, S.Sirri -- 0000-0003-2878-6968, Ondokuz Mayıs Üniversitesi
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Insecticides
Antioxidant
Health
Toxicology and Mutagenesis
medicine.medical_treatment
010501 environmental sciences
Toxicology
medicine.disease_cause
01 natural sciences
chlorpyrifos
Antioxidants
Rats
Sprague-Dawley
chemistry.chemical_compound
Organophosphate Poisoning
Organophosphate
oxidative stress
chemistry.chemical_classification
Neurons
omega-3 fatty acids
biology
Behavior
Animal
Glutathione peroxidase
Brain
docosahexaenoic acid
Malondialdehyde
pesticide toxicology
Neuroprotective Agents
Liver
Docosahexaenoic acid
Toxicity
Female
Chlorpyrifos
Locomotion
Body Temperature Regulation
medicine.medical_specialty
Docosahexaenoic Acids
Injections
Subcutaneous
03 medical and health sciences
Internal medicine
Fatty Acids
Omega-3
Weight Loss
medicine
Animals
0105 earth and related environmental sciences
Cholinesterase
Public Health
Environmental and Occupational Health
Oxidative Stress
030104 developmental biology
Endocrinology
chemistry
Dietary Supplements
biology.protein
Cholinesterase Inhibitors
Oxidative stress
Biomarkers
Zdroj: Toxicology and industrial health. 34(2)
ISSN: 1477-0393
Popis: WOS: 000425176300001
PubMed ID: 29141517
In this study, we aimed to study the possible preventive effect of docosahexaenoic acid (DHA), a dietary omega-3 fatty acid, on toxicity caused by chlorpyrifos (CPF). Six groups of Sprague Dawley rats (200-250 g) consisting of equal numbers of males and females (n = 8) were assigned to study. The rats were orally given for 5 days. The control group was administered pure olive oil, which was the vehicle for CPF. The CPF challenge groups were administered oral physiological saline, pure olive oil, or DHA (50, 100 and 400 mg/kg dosages) for 5 days. The animals were weighed on the sixth day and then administered CPF (279 mg/kg, subcutaneously). The rats were weighed again 24 h following CPF administration. The body temperatures and locomotor activities of the rats were also measured. Blood samples, brain and liver tissues were collected for biochemical, histopathological and immunohistochemical examinations. A comparison with the control group demonstrated that CPF administration increased malondialdehyde (MDA) levels in blood, brain and liver, while it reduced catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) concentrations (p < 0.05-0.001). Advanced oxidation protein products (AOPPs) increased only in the brain (p < 0.001). DHA reduced these changes in MDA and AOPP values (p < 0.05-0.001), while it increased CAT, SOD and GPx concentrations (p < 0.05-0.001). Similarly, DHA prevented the decreases in body weight, body temperature and locomotor activities caused by CPF at 100 mg/kg and 400 mg/kg dosages (p < 0.05-0.001). Similar to the physiological and biochemical changes, the histopathological damage scores, which increased with CPF (p < 0.05-0.01), decreased at all three dosages of DHA (p < 0.05-0.01). Our findings suggest that DHA, by supporting the antioxidant mechanism, reduces toxicity caused by CPF.
Ondokuz Mayis University Research Fund [PYO. TIP. 1901.13.013]
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by Ondokuz Mayis University Research Fund. PYO. TIP. 1901.13.013.
Databáze: OpenAIRE
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