Best temperature for static liver graft storage is 1°C
| Autor: | Laurent Hannoun, Eric Savier, Jean-Claude Chaumeil, Nathalie Neveux, Christine Charrueau, Luc Cynober |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Cold storage chemistry.chemical_element Liver transplantation Oxygen Rats Sprague-Dawley Oxygen Consumption Internal medicine medicine Animals Bile Viaspan L-Lactate Dehydrogenase biology Temperature Alanine Transaminase Organ Preservation Liver Transplantation Rats Liver graft Transplantation Endocrinology chemistry Biochemistry biology.protein Vascular Resistance Surgery Creatine kinase Liver function |
| Zdroj: | Journal of Surgical Research. 180:322-329 |
| ISSN: | 0022-4804 |
| DOI: | 10.1016/j.jss.2012.04.046 |
| Popis: | The best storage temperature in liver transplantation remains an unsolved question.After storage for 24h in University of Wisconsin solution at +4°C, +1°C, or -0.5°C, rat livers were subjected, or not, to 15min of warm ischemia, rinsed with Ringer lactate, and subsequently reperfused with oxygenated Krebs-Henseleit buffer.In the presence of warm ischemia, for livers stored at +4°C, creatine kinase (CK) peaked at 21±5IUg(-1)h(-1), hepatic resistance at 34,700±1500dynscm(-5), bile flow reached 18±4μLg(-1)h(-1) after 10min, and oxygen consumption stabilized at about 25μmolg(-1)h(-1) after 20min. When livers were stored at +1°C, CK and hepatic resistance were lowered, bile production was 33±6μLg(-1)h(-1) (P0.05 versus +4°C), and oxygen consumption was 105±10μmolg(-1)h(-1) (P0.001). For livers stored at-0.5°C, results were not statistically different from those of the +1°C group except for bile flow, which was significantly lower. Without warm ischemia, the peak of CK (P=0.015) and the peak hepatic resistance (P0.001) of the +4°C group were significantly increased compared with the +1°C or -0.5°C groups. However, no difference in bile flow or oxygen consumption was observed. The number of trypan blue-positive nonparenchymal cells (P=0.003) and the gain in liver weight during the reperfusion (P=0.015) were minimal after storage at +1°C.Static storage at +1°C improved liver function compared with +4°C or -0.5°C. Main beneficial effect was observed with parameters reflecting sinusoidal cells injury. |
| Databáze: | OpenAIRE |
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