Highly Stable Passive Wireless Sensor for Protease Activity Based on Fatty Acid-Coupled Gelatin Composite Films
| Autor: | Javier Sotres, Tautgirdas Ruzgas, Palraj Kalimuthu, Juan F. Gonzalez-Martinez |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Proteases
Support Vector Machine food.ingredient medicine.medical_treatment Medical Laboratory and Measurements Technologies Composite number Nanotechnology 010402 general chemistry 01 natural sciences Gelatin Article Analytical Chemistry chemistry.chemical_compound food medicine Wireless Medicinsk laboratorie- och mätteknik chemistry.chemical_classification Protease Aqueous medium business.industry Fatty Acids 010401 analytical chemistry Caprylic acid Fatty acid 0104 chemical sciences Aspergillus chemistry Quartz Crystal Microbalance Techniques business Wireless Technology Peptide Hydrolases |
| Zdroj: | Analytical Chemistry |
| ISSN: | 1520-6882 0003-2700 |
| DOI: | 10.1021/acs.analchem.0c02153 |
| Popis: | Proteases are often used as biomarkers of many pathologies as well as of microbial contamination and infection. Therefore, extensive efforts are devoted to the development of protease sensors. Some applications would benefit from wireless monitoring of proteolytic activity at minimal cost, e.g., sensors embedded in care products like wound dressings and diapers to track wound and urinary infections. Passive (batteryless) and chipless transponders stand out among wireless sensing technologies when low cost is a requirement. Here, we developed and extensively characterized a composite material that is biodegradable but still highly stable in aqueous media, whose proteolytic degradation could be used in these wireless transponders as a transduction mechanism of proteolytic activity. This composite material consisted of a cross-linked gelatin network with incorporated caprylic acid. The digestion of the composite when exposed to proteases results in a change of its resistivity, a quantity that can be wirelessly monitored by coupling the composite to an inductor-capacitor resonator, i.e., an antenna. We experimentally proved this wireless sensor concept by monitoring the presence of a variety of proteases in aqueous media. Moreover, we also showed that detection time follows a relationship with protease concentration, which enables quantification possibilities for practical applications. |
| Databáze: | OpenAIRE |
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