Oligonucleotide sensor based on magnetic capture and photoligation of upconverting nanoparticles in solid surfaces
| Autor: | Diego Mendez-Gonzalez, Sonia Melle, Ana Egatz-Gomez, Juan L. Mendez-Gonzalez, Oscar G. Calderón, Jorge Rubio-Retama, Marco Laurenti, Pedro P. Silva-Ibáñez, Fernando Valiente-Dies, Elena Díaz |
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| Přispěvatelé: | Agencia Estatal de Investigación (España), Comunidad de Madrid, Universidad Complutense de Madrid, Comisión Nacional de Investigación Científica y Tecnológica (Chile), European Commission |
| Rok vydání: | 2021 |
| Předmět: |
Luminescence
Oligonucleotides Nanoparticle DNA Single-Stranded Sequence (biology) 02 engineering and technology Biosensing Techniques 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Colloid and Surface Chemistry Upconverting nanoparticles Humans Técnicas de la imagen Magnetic microparticles Óptica Detection limit DNA detection Oligonucleotide Chemistry Diagnóstico por imagen y medicina nuclear Física 021001 nanoscience & nanotechnology Combinatorial chemistry 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Covalent bond Partículas Nanoparticles 0210 nano-technology DNA |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 11 pags., 7 figs., 1 tab. In this work, we present a luminescence platform that can be used as point of care system for determining the presence and concentration of specific oligonucleotide sequences. This sensor exhibited a limit of detection as low as 50 fM by means of: (i) the use of single-stranded DNA (ssDNA) functionalized magnetic microparticles that captured and concentrated ssDNA-upconverting nanoparticles (ssDNA-UCNPs) on a solid support, when the target sequence (miR-21-5p DNA-analogue) was in the sample, and (ii) a photoligation reaction that covalently linked the ssDNA-UCNPs and the ssDNA magnetic microparticles, allowing stringent washes. The presented sensor showed a similar limit of detection when the assays were conducted in samples containing total miRNA extracted from human serum, demonstrating its suitability for detecting small specific oligonucleotide sequences under real-like conditions. The strategy of combining UCNPs, magnetic microparticles, and a photoligation reaction provides new insight into low-cost, rapid, and ultra-sensitive detection of oligonucleotide sequences. This work was supported by Ministerio de Economía y Competi- tividad (MAT2017-83111R and PID2019-106820RB-C21), Ministerio de Ciencia, Innovacion, y Universidades (MICINN, RTI2018-094859) and Comunidad de Madrid (B2017/BMD-3867 RENIM-CM) co-financed by European Structural and Investment Fund. D.M.G. thanks UCM-Santander for a predoctoral contract (CT17/17- CT18/17) and a Postdoctoral Orientation Period (POP) contract. P. S. I. thanks CONICYT for a predoctoral contract (N° 21161052). F. V. D. thanks to European Commission for a YEI program contract (PEJD-2018-PRE/IND-9118). TEM images were obtained at the National Center for Electron Microscopy (UCM, Madrid) facility |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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