Effects of T-2 toxin on the regulation of steroidogenesis in mouse Leydig cells
| Autor: | Jian Ying Yang, Yong Fa Zhang, Ai Min Liang, Xiangfeng Kong, Kai Wang Ma, Gui Ping Guan, Yuan Xiao Li, Guang Da Li, Xue Fei Bai |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male endocrine system medicine.medical_specialty 17-Hydroxysteroid Dehydrogenases Health Toxicology and Mutagenesis Cell Dehydrogenase Centrifugation 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences Human chorionic gonadotropin 03 medical and health sciences Mice Transcription (biology) Internal medicine medicine Animals RNA Messenger Cells Cultured 0105 earth and related environmental sciences chemistry.chemical_classification biology Toxin Cholesterol side-chain cleavage enzyme Public Health Environmental and Occupational Health Cytochrome P450 Leydig Cells Phosphoproteins Molecular biology T-2 Toxin 030104 developmental biology Endocrinology Enzyme medicine.anatomical_structure chemistry biology.protein |
| Zdroj: | Toxicology and industrial health. 32(10) |
| ISSN: | 1477-0393 |
| Popis: | T-2 toxin is one of the mycotoxins, a group of type A trichothecenes produced by several fungal genera including Fusarium species, which may lead to the decrease of testosterone secretion in primary Leydig cells derived from mouse testis. The previous study demonstrated T-2 toxin decrease the testosterone biosynthesis in the primary Leydig cells derived from the mouse testis directly. In this study, we further examined the direct biological effects of T-2 toxin on the process of steroidogenesis, primarily in Leydig cells of mice. Leydig cells of mature mouse were purified by Percoll gradient centrifugation and the cell purity was determined by 3β-hydroxysteroid dehydrogenase (3β-HSD) staining. To examine the decrease in T-2 toxin-induced testosterone secretion, we measured the transcription level of three key steroidogenic enzymes including 3β-HSD-1, cytochrome P450 side-chain cleavage (P450scc) enzyme, and steroidogenic acute regulatory (StAR) protein in T-2 toxin/human chorionic gonadotropin (hCG) co-treated cells. Our previous study showed that T-2 toxin (10−7, 10−8, and 10−9 M) significantly suppressed hCG (10 ng/ml)-induced testosterone secretion. The studies demonstrated that the suppressive effect is correlated with a decrease in the level of transcription of 3β-HSD-1, P450scc, and StAR ( p < 0.05). |
| Databáze: | OpenAIRE |
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