The detection of gastric cancer cells in intraoperative peritoneal lavage using the reverse transcription?loop-mediated isothermal amplification method
| Autor: | Yasuhiro Torashima, Seiya Susumu, Susumu Eguchi, Tamotsu Kuroki, Ken Taniguchi, Kengo Kanetaka, Akira Yoneda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty genetic structures Loop-mediated isothermal amplification Gastroenterology Sensitivity and Specificity Cytokeratin Intraoperative Period Stomach Neoplasms Internal medicine Cytology medicine Adjuvant therapy Biomarkers Tumor Humans Clinical significance RNA Messenger Peritoneal lavage Reverse Transcription Loop-mediated Isothermal Amplification Aged RT-LAMP Keratin-19 Univariate analysis business.industry Reverse Transcriptase Polymerase Chain Reaction Carcinoma Cancer DNA Neoplasm Middle Aged medicine.disease Neoplastic Cells Circulating eye diseases Molecular Diagnostic Techniques Surgery Female sense organs business Gastric cancer |
| Zdroj: | Journal of Surgical Research. 187(1):e1-e6 |
| ISSN: | 0022-4804 |
| Popis: | Introduction: To detect a small number of malignant cells, we used a highly sensitive detection system that measures the expression levels of cytokeratin (CK) 19 messenger RNA by reverse transcription-loop-mediated isothermal amplification (RT-LAMP). Materials and methods: We evaluated the clinical relevance of our novel diagnostic method with an RT-LAMP assay using CK19 as a target gene for the detection of free cancer cells in peritoneal lavage and assessed the clinical significance of the molecular diagnosis by survival analysis and frequency of recurrence, with a median follow-up period of 39 mo. We observed 52 patients with gastric cancer who underwent gastrectomy, bypass operation, and exploratory laparotomy. Results: Those 52 patients, who were subjected to both RT-LAMP and cytologic examination, were divided into the following three groups: (1) patients positive by cytology and RT-LAMP (CY+/LAMP+) (n = 9), (2) patients positive by LAMP and negative by cytology (CY-/LAMP+) (n = 12), and (3) patients negative by both cytology and LAMP (CY-/LAMP-) (n = 31). All patients with simultaneous peritoneal dissemination and positive cytology were positive on RT-LAMP. The results of RT-LAMP were statistically significant for recurrence by univariate analysis (P < 0.005). Cytology-positive cases had a very poor prognosis, and RT-LAMP-positive cases had a worse prognosis than RT-LAMP-negative cases. Conclusions: Our findings suggest that CK19 RT-LAMP would be useful as an intraoperative diagnostic modality to detect patients with a high risk of recurrence even after clinically curative surgery, who thus require proper adjuvant therapy. Journal of Surgical Research, 187(1), pp.e1-e6; 2014 |
| Databáze: | OpenAIRE |
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